Evidence map›Paper›PMID 33865857›Full record

ArticleThe Journal of biological chemistry

DUSP7 regulates the activity of ERK2 to promote proper chromosome alignment during cell division.

Xiao Guo, Ivan Ramirez, Yenni A Garcia, Erick F Velasquez, Ankur A Gholkar, Whitaker Cohn, Julian P Whitelegge, Bobby Tofig, Robert Damoiseaux, Jorge Z Torres

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.9field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Xiao GuoDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, USA.
Ivan RamirezDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, USA.
Yenni A GarciaDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, USA.
Erick F VelasquezDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, USA.
Ankur A GholkarDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, USA.
Whitaker CohnPasarow Mass Spectrometry Laboratory, The Jane and Terry Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California, USA.
Julian P WhiteleggePasarow Mass Spectrometry Laboratory, The Jane and Terry Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California, USA; Molecular Biology Institute, University of California, Los Angeles, California, USA; Jonsson Comprehensive Cancer Center, University of California, Los Angeles, California, USA.
Bobby TofigCalifornia NanoSystems Institute, University of California, Los Angeles, California, USA.
Robert DamoiseauxCalifornia NanoSystems Institute, University of California, Los Angeles, California, USA; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, California, USA.
Jorge Z TorresDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, USA; Molecular Biology Institute, University of California, Los Angeles, California, USA; Jonsson Comprehensive Cancer Center, University of California, Los Angeles, California, USA. Electronic address: torres@chem.ucla.edu.
University of California, Los Angeles · USCalifornia NanoSystems Institute · US

Funding

Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Robert Damoiseaux · 1985 to 2026
$134.5M
Transgenic & Knock-out MouseP30DK063491 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI MILES Frome WILKINSON · 2003 to 2026
$40.4M
TUMOR CELL SURFACES AND CHROMOSOMEST32CA009056 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Gay M Crooks, David A. Nathanson · 1985 to 2026
$10.5M
Investigating the Cell Division MachineryR35GM139539 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TORRES, JORGE · 2021 to 2025
$2.2M
Novel Regulatory Factors of the Spindle Assembly CheckpointR01GM117475 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TORRES, JORGE · 2016 to 2020
$1.7M
Howard Hughes Medical InstituteNCI NIH HHS P30 CA016042NCI NIH HHS T32 CA009056NIDDK NIH HHS P30 DK063491NIGMS NIH HHS R01 GM117475NIGMS NIH HHS R35 GM139539
6 · The paper itself

Abstract

Human cell division is a highly regulated process that relies on the accurate capture and movement of chromosomes to the metaphase plate. Errors in the fidelity of chromosome congression and alignment can lead to improper chromosome segregation, which is correlated with aneuploidy and tumorigenesis. These processes are known to be regulated by extracellular signal-regulated kinase 2 (ERK2) in other species, but the role of ERK2 in mitosis in mammals remains unclear. Here, we have identified the dual-specificity phosphatase 7 (DUSP7), known to display selectivity for ERK2, as important in regulating chromosome alignment. During mitosis, DUSP7 bound to ERK2 and regulated the abundance of active phospho-ERK2 through its phosphatase activity. Overexpression of DUSP7, but not catalytically inactive mutants, led to a decrease in the levels of phospho-ERK2 and mitotic chromosome misalignment, while knockdown of DUSP7 also led to defective chromosome congression that resulted in a prolonged mitosis. Consistently, knockdown or chemical inhibition of ERK2 or chemical inhibition of the MEK kinase that phosphorylates ERK2 led to chromosome alignment defects. Our results support a model wherein MEK-mediated phosphorylation and DUSP7-mediated dephosphorylation regulate the levels of active phospho-ERK2 to promote proper cell division.

Indexed as

MitosisChromosomes, HumanDual-Specificity PhosphatasesHCT116 CellsHeLa CellsHumansMAP Kinase Kinase KinasesMitogen-Activated Protein Kinase 1MutationPhosphorylationDual-Specificity PhosphatasesDUSP7 protein, humanMAPK1 protein, humanMAP Kinase Kinase KinasesMitogen-Activated Protein Kinase 1cell divisionchromosome alignmentdual-specificity phosphatase 7 (DUSP7)extracellular signal-regulated kinase 2 (ERK2)mitogen-activated protein kinases (MAPK)mitosis

Identifiers

PMID33865857
PMCPMC8131738
OpenAlexW3153321041

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.