Evidence map›Paper›PMID 33865380›Full record

ArticleMicrobial cell factories2021

Lyophilized alginate-based microspheres containing Lactobacillus fermentum D12, an exopolysaccharides producer, contribute to the strain's functionality in vitro.

Katarina Butorac, Jasna Novak, Barbara Bellich, Lucrecia C Terán, Martina Banić, Andreja Leboš Pavunc, Slaven Zjalić, Paola Cescutti, Jagoda Šušković, Blaženka Kos

Open access · goldAbstract read
In one paragraph

Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
3.4field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 32 citations in OpenAlex.

  1. Article
  2. Article
  3. Genomic Insights and Inactivation Strategies forFoods (Basel, Switzerland) · 2026
    Article
  4. In Vivo Evaluation of the Effect ofInternational journal of molecular sciences · 2026
    Article
  5. Article
  6. Article
  7. Article
  8. In Vitro Analysis of Probiotic Properties Related to the Adaptation ofInternational journal of molecular sciences · 2025
    Article
  9. Probiotic characteristics and whole genome sequencing ofFrontiers in veterinary science · 2025
    Article
  10. Preparation and In Vitro Characterization ofJournal of functional biomaterials · 2024
    Article
  11. Evaluation of the Probiotic Properties ofFood technology and biotechnology · 2023
    Article
  12. Screening of exopolysaccharide-producingFrontiers in microbiology · 2023
    Article
  13. Article
  14. Article
  15. Biotechnology reports (Amsterdam, Netherlands) · 2022
    Article
  16. Foods (Basel, Switzerland) · 2022
    Article
  17. Optimization of growth ofFrontiers in bioengineering and biotechnology · 2022
    Article
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Katarina ButoracLaboratory of Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, Zagreb, Croatia.
Jasna NovakLaboratory of Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, Zagreb, Croatia. jasna.novak@pbf.unizg.hr.ORCID http://orcid.org/0000-0003-1374-1097
Barbara BellichDepartment of Life Sciences, University of Trieste, Via Licio Giorgieri 1, Bdg. C11, 34127, Trieste, Italy.
Lucrecia C TeránDepartment of Life Sciences, University of Trieste, Via Licio Giorgieri 1, Bdg. C11, 34127, Trieste, Italy.
Martina BanićLaboratory of Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, Zagreb, Croatia.
Andreja Leboš PavuncLaboratory of Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, Zagreb, Croatia.
Slaven ZjalićDepartment of Ecology, Agronomy and Aquaculture, University of Zadar, Trg Kneza Višeslava 9, 23000, Zadar, Croatia.
Paola CescuttiDepartment of Life Sciences, University of Trieste, Via Licio Giorgieri 1, Bdg. C11, 34127, Trieste, Italy.
Jagoda ŠuškovićLaboratory of Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, Zagreb, Croatia.
Blaženka KosLaboratory of Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, Zagreb, Croatia.
University of Zagreb · HRUniversity of Trieste · ITUniversity of Zadar · HR

Funding

Hrvatska Zaklada za Znanost IP-2014-09-7009Hrvatska Zaklada za Znanost IP-2019-04-2237Sveučilište u Zagrebu 000700007439
6 · The paper itself

Abstract

Lactobacillus (Limosilactobacillus) fermentum D12 is an exopolysaccharide (EPS) producing strain whose genome contains a putative eps operon. Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis. These included the genes required for EPS biosynthesis such as epsA, B, C, D and E, also gt, wzx, and wzy and those involved in the activation of the precursor molecules galE, galT and galU. Both the biosynthesis and export mechanism of EPS were proposed based on functional annotation. When grown on MRS broth with an additional 2% w/v glucose, L. fermentum D12 secreted up to 200 mg/L of a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM). Structural information obtained by

Indexed as

MicrospheresAlginatesLimosilactobacillus fermentumPolysaccharides, BacterialProbioticsAlginatesPolysaccharides, BacterialEps clusterExopolysaccharidesLimosilactobacillus (Lactobacillus) fermentumPrebioticProbiotic

Identifiers

PMID33865380
PMCPMC8052780
OpenAlexW3152539884

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.