ArticleScientific reports2021
Self-organization and culture of Mesenchymal Stem Cell spheroids in acoustic levitation.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Influence of gravity variations on the activity of neuronal spheroids in an acoustic trap.NPJ microgravity · 2026Article
- Illumination optimization and low-power trapping of Limnospira indica PCC 8005 using bulk acoustic waves in microgravity.NPJ microgravity · 2026Article
- On-chip particle levitation and micromanipulation using bulk acoustic waves.Lab on a chip · 2025Article
- Three-dimensional spheroid models for cardiovascular biology and pathology.Mechanobiology in medicine · 2025Review
- Mass Production of Uniform Embryoid Bodies by Acoustic Standing Waves.Small methods · 2025Article
- An acoustic levitation platform for high-content histological analysis of 3D tissue culture.Lab on a chip · 2025Article
- Biological Acoustic Levitation and Its Potential Application for Microgravity Study.Bioengineering (Basel, Switzerland) · 2025Review
- A multi-stage weakly supervised design for spheroid segmentation to explore mesenchymal stem cell differentiation dynamics.BMC bioinformatics · 2025Article
- 3D Culture of MSCs for Clinical Application.Bioengineering (Basel, Switzerland) · 2024Review
- Acoustic virtual 3D scaffold for direct-interacting tumor organoid-immune cell coculture systems.Science advances · 2024Article
- Improved functionality of hepatic spheroids cultured in acoustic levitation compared to existing 2D and 3D models.Scientific reports · 2024Article
- Ultrasound-induced reorientation for multi-angle optical coherence tomography.Nature communications · 2024Article
- AnMaterials today. Bio · 2023Article
- Acoustic and Magnetic Stimuli-Based Three-Dimensional Cell Culture Platform for Tissue Engineering.Tissue engineering and regenerative medicine · 2023Review
- Subaqueous free-standing 3D cell culture system for ultrafast cell compaction, mechano-inductive immune control, and improving therapeutic angiogenesis.Bioengineering & translational medicine · 2023Article
- Therapeutic strategies of three-dimensional stem cell spheroids and organoids for tissue repair and regeneration.Bioactive materials · 2023Review
- High-throughput fabrication of cell spheroids with 3D acoustic assembly devices.International journal of bioprinting · 2023Article
- Active matter in space.NPJ microgravity · 2022Review
- Ultrasound-Responsive Systems as Components for Smart Materials.Chemical reviews · 2022Review
- Potential of the acoustic micromanipulation technologies for biomedical research.Biomicrofluidics · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years, 3D cell culture models such as spheroid or organoid technologies have known important developments. Many studies have shown that 3D cultures exhibit better biomimetic properties compared to 2D cultures. These properties are important for in-vitro modeling systems, as well as for in-vivo cell therapies and tissue engineering approaches. A reliable use of 3D cellular models still requires standardized protocols with well-controlled and reproducible parameters. To address this challenge, a robust and scaffold-free approach is proposed, which relies on multi-trap acoustic levitation. This technology is successfully applied to Mesenchymal Stem Cells (MSCs) maintained in acoustic levitation over a 24-h period. During the culture, MSCs spontaneously self-organized from cell sheets to cell spheroids with a characteristic time of about 10 h. Each acoustofluidic chip could contain up to 30 spheroids in acoustic levitation and four chips could be ran in parallel, leading to the production of 120 spheroids per experiment. Various biological characterizations showed that the cells inside the spheroids were viable, maintained the expression of their cell surface markers and had a higher differentiation capacity compared to standard 2D culture conditions. These results open the path to long-time cell culture in acoustic levitation of cell sheets or spheroids for any type of cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.