ArticleNPJ biofilms and microbiomes2021
Experimental parameters defining ultra-low biomass bioaerosol analysis.
Article in NPJ biofilms and microbiomes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Sampling and analysis methods for detecting and monitoring airborne plant pathogens and fungal diversity.Microbiology (Reading, England) · 2026Review
- Classroom microbiome signatures of pest management associate with reduced asthma symptoms.The Journal of allergy and clinical immunology · 2026Article
- Disinfection of hospital sink drains enriches pseudomonadota and efflux pump-mediated antibiotic resistance in reestablished biofilms.Nature communications · 2026Article
- Article
- Adaptation of the multiplexed CRISPR-Cas13 CARMEN RVP assay for longitudinal detection of respiratory pathogens from air samples.Applied and environmental microbiology · 2026Article
- Spatial and temporal patterns of public transit aerobiomes.Microbiome · 2026Article
- Metagenomic profiling of airborne microbial communities from aircraft filters and face masks.Microbiome · 2025Article
- Disinfection of Hospital Sink Drains Enriches Pseudomonadota and Efflux Pump-Mediated Antibiotic Resistance in Reestablished Biofilms.Research square · 2025Article
- Adaptation of the multiplexed CRISPR-Cas13 CARMEN RVP assay for longitudinal detection of respiratory pathogens from air samples.medRxiv : the preprint server for health sciences · 2025Article
- Article
- From air to insight: the evolution of airborne DNA sequencing technologies.Microbiology (Reading, England) · 2025Review
- Comparison of airborne bacterial communities in PM2.5 between a dry-season haze period and a wet-season non-haze period in thailand.Scientific reports · 2025Article
- Analysis of metagenomic data.Nature reviews. Methods primers · 2025Article
- Metataxonomics study of dental bioaerosols affected by waterline disinfection.BMC oral health · 2024Article
- Residential exposure toThe European respiratory journal · 2024Article
- Multi-scenario surveillance of respiratory viruses in aerosols with sub-single-copy spatial resolution.Nature communications · 2024Article
- Microbial richness and air chemistry in aerosols above the PBL confirm 2,000-km long-distance transport of potential human pathogens.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Sampling efficiency and nucleic acid stability during long-term sampling with different bioaerosol samplers.Environmental monitoring and assessment · 2024Article
- Fungal Spore Richness and Abundance of Allergenic Taxa: Comparing a Portable Impactor and Passive Trap Indoors and Outdoors in an Urban Setting.Microbial ecology · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
24 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Investigation of the microbial ecology of terrestrial, aquatic and atmospheric ecosystems requires specific sampling and analytical technologies, owing to vastly different biomass densities typically encountered. In particular, the ultra-low biomass nature of air presents an inherent analytical challenge that is confounded by temporal fluctuations in community structure. Our ultra-low biomass pipeline advances the field of bioaerosol research by significantly reducing sampling times from days/weeks/months to minutes/hours, while maintaining the ability to perform species-level identification through direct metagenomic sequencing. The study further addresses all experimental factors contributing to analysis outcome, such as amassment, storage and extraction, as well as factors that impact on nucleic acid analysis. Quantity and quality of nucleic acid extracts from each optimisation step are evaluated using fluorometry, qPCR and sequencing. Both metagenomics and marker gene amplification-based (16S and ITS) sequencing are assessed with regard to their taxonomic resolution and inter-comparability. The pipeline is robust across a wide range of climatic settings, ranging from arctic to desert to tropical environments. Ultimately, the pipeline can be adapted to environmental settings, such as dust and surfaces, which also require ultra-low biomass analytics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.