ArticleApplied and environmental microbiology2021
Pyruvate Production by Escherichia coli by Use of Pyruvate Dehydrogenase Variants.
Article in Applied and environmental microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- IMACulate(DE3), an E. coli Strain for High Purity His-Tagged Protein Purifications.Biotechnology and bioengineering · 2026Article
- Measuring bacterial oxygen consumption rate to probe metabolic signature and antimicrobial susceptibility.European biophysics journal : EBJ · 2026Article
- Functional remodeling of the gut microbiome and metabolome in primary idiopathic male infertility.BMC microbiology · 2026Article
- Antibacterial Effect and Mechanism of Chelerythrine onMicroorganisms · 2025Article
- Low-biomass pyruvate production with engineered Vibrio natriegens is accompanied by parapyruvate formation.Microbial cell factories · 2025Article
- Reprogramming aerobic metabolism mitigates Streptococcus pyogenes tissue damage in a mouse necrotizing skin infection model.Nature communications · 2025Article
- Advancements in metabolic engineering: unlocking the potential of key organic acids for sustainable industrial applications.Frontiers in bioengineering and biotechnology · 2025Review
- Substitute Yeast Extract While Maintaining Performance: Showcase Amorpha-4,11-Diene Production.Microbial biotechnology · 2024Article
- Isolation and Characterization of High-Temperature-Tolerant Mutants ofMicroorganisms · 2024Article
- Catabolism of 2-keto-3-deoxy-galactonate and the production of its enantiomers.Applied microbiology and biotechnology · 2024Review
- Citrate synthase variants improve yield of acetyl-CoA derived 3-hydroxybutyrate in Escherichia coli.Microbial cell factories · 2024Article
- Genomic and transcriptomics analysis reveal putative secreted proteins expressed ofFrontiers in veterinary science · 2024Article
- Escherichia coli BL21(DE3) optimized deletion mutant as the host for whole-cell biotransformation of N‑acetyl‑D‑neuraminic acid.Biotechnology letters · 2023Article
- Metabolic engineering of fast-growing Vibrio natriegens for efficient pyruvate production.Microbial cell factories · 2023Article
- Advances in the optimization of central carbon metabolism in metabolic engineering.Microbial cell factories · 2023Review
- Innova 2020: A Follow-Up Study of the Fecal Microbiota of Infants Using a Novel Infant Formula between 6 Months and 12 Months of Age.International journal of molecular sciences · 2023Article
- Article
- Metabolic Engineering of Microorganisms to Produce Pyruvate and Derived Compounds.Molecules (Basel, Switzerland) · 2023Review
- Metabolic engineering using acetate as a promising building block for the production of bio-based chemicals.Engineering microbiology · 2022Review
- Nutrient-Limited Operational Strategies for the Microbial Production of Biochemicals.Microorganisms · 2022Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Altering metabolic flux at a key branch point in metabolism has commonly been accomplished through gene knockouts or by modulating gene expression. An alternative approach to direct metabolic flux preferentially toward a product is decreasing the activity of a key enzyme through protein engineering. In Escherichia coli, pyruvate can accumulate from glucose when carbon flux through the pyruvate dehydrogenase complex is suppressed. Based on this principle, 16 chromosomally expressed AceE variants were constructed in E. coli C and compared for growth rate and pyruvate accumulation using glucose as the sole carbon source. To prevent conversion of pyruvate to other products, the strains also contained deletions in two nonessential pathways: lactate dehydrogenase (
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