Evidence map›Paper›PMID 33861424›Full record

ArticleEnvironmental science and pollution research international2021

Effects of allelochemical artemisinin in Artemisia annua on Microcystis aeruginosa: growth, death mode, and microcystin-LR changes.

Lixiao Ni, Hanqi Wu, Cunhao Du, Xianglan Li, Yan Li, Chu Xu, Peifang Wang, Shiyin Li, Jianhua Zhang, Xuqing Chen

Abstract read
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In one paragraph

Article in Environmental science and pollution research international, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.5field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Lixiao NiKey Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, MOE, School of Environment, Hohai University, Nanjing, 210098, China.
Hanqi WuKey Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, MOE, School of Environment, Hohai University, Nanjing, 210098, China.
Cunhao DuKey Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, MOE, School of Environment, Hohai University, Nanjing, 210098, China.
Xianglan LiKey Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, MOE, School of Environment, Hohai University, Nanjing, 210098, China.
Yan LiKey Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, MOE, School of Environment, Hohai University, Nanjing, 210098, China.
Chu XuKey Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, MOE, School of Environment, Hohai University, Nanjing, 210098, China.
Peifang WangKey Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, MOE, School of Environment, Hohai University, Nanjing, 210098, China.
Shiyin LiSchool of Environment, Nanjing Normal University, Nanjing, 210097, China. lishiyin@njnu.edu.cn.ORCID http://orcid.org/0000-0002-1550-4258
Jianhua ZhangJiangsu Provincial Water Conservancy Department, Nanjing, 210029, China.
Xuqing ChenCyanobacteria Management Office, Wuxi, 214071, China.
Hohai University · CNJiangsu Provincial Water Survey & Design Institute (China) · CNNanjing Normal University · CN

Funding

the National Natural Science Foundation No. 51779079the National Natural Science Foundation of China (CN) NO.51979137the Natural Science Foundation of Jiangsu Province No. BK20181313
6 · The paper itself

Abstract

To investigate the effects of an allelochemical artemisinin extracted from Artemisia annua (A. annua) on cell growth, death mode, and microcystin-LR (MC-LR) changes of Microcystis aeruginosa (M. aeruginosa), a series of morphological and biochemical characteristics were studied. The results showed that artemisinin could inhibit the growth of M. aeruginosa and reduce the content of phycobiliprotein. Under the allelopathy of artemisinin, algae cells deformed due to swelling, which caused cell membranes to rupture and cell contents to leak. FDA/PI double-staining results showed that 15.10-94.90% of algae cells experienced the death mode of necrosis-like. Moreover, there were 8.35-14.50% of algae cells undergoing programmed cell death, but their caspase-3-like protease activity remained unchanged, which may mean that algae cells were not experiencing caspase-dependent apoptosis under artemisinin stress. Attacked by artemisinin directly, both intracellular and extracellular MC-LR increased sharply with the upregulation of mcyB, mcyD, and mcyH. The upregulation multiple of mcyH suggested that M. aeruginosa could accelerate transportation of algal toxin under adverse conditions of artemisinin. Artemisinin not only can inhibit the growth of M. aeruginosa but it also causes the accelerated release and increase of microcystin-LR. These imply that the application of artemisinin should be reconsidered in practical water bodies.

Indexed as

Artemisia annuaArtemisininsMicrocystisMarine ToxinsMicrocystinsPheromonesArtemisininscyanoginosin LRMarine ToxinsMicrocystinsPheromonesArtemisininDeath modeMicrocystin-LRMicrocystis aeruginosaNecrosis-like

Identifiers

PMID33861424
OpenAlexW3156087290

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.