ArticleLab on a chip2021
Measuring barrier function in organ-on-chips with cleanroom-free integration of multiplexable electrodes.
Article in Lab on a chip, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Development of a Human 3D Immune-Competent Neurovascular Model Enabling Time-Resolved Monitoring of Neuroinflammatory Dynamics and Neuroimmune Interactions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Automated culture and monitoring of a high-throughput human heart-on-a-chip.bioRxiv : the preprint server for biology · 2026Article
- Standardizing TEER Measurements in Blood-Brain Barrier-on-Chip Systems: A Systematic Review of Electrode Designs and Configurations.Biomimetics (Basel, Switzerland) · 2026Review
- Mechano-Organ-on-Chip for Cancer Research.International journal of molecular sciences · 2026Review
- Sensors Manufacturing on a 3D-Printed Transwell-Based Hybrid Organ-on-a-Chip for Non-Invasive Real-Time Biological Barrier Resistance Monitoring.ACS biomaterials science & engineering · 2025Article
- Current and Future Cornea Chip Models for Advancing Ophthalmic Research and Therapeutics.Advanced biology · 2025Review
- Modeling reproductive and pregnancy-associated tissues using organ-on-chip platforms: challenges, limitations, and the high throughput data frontier.Frontiers in bioengineering and biotechnology · 2025Review
- Standardizing designed and emergent quantitative features in microphysiological systems.Nature biomedical engineering · 2024Review
- Establishment and evaluation of on-chip intestinal barrier biosystems based on microfluidic techniques.Materials today. Bio · 2024Review
- Innovative electrode and chip designs for transendothelial electrical resistance measurements in organs-on-chips.Lab on a chip · 2024Review
- Humanized brain organoids-on-chip integrated with sensors for screening neuronal activity and neurotoxicity.Mikrochimica acta · 2024Review
- Bio-Microfabrication of 2D and 3D Biomimetic Gut-on-a-Chip.Micromachines · 2023Review
- Gut-on-a-Chip Research for Drug Development: Implications of Chip Design on Preclinical Oral Bioavailability or Intestinal Disease Studies.Biomimetics (Basel, Switzerland) · 2023Review
- Article
- Building Blood Vessel Chips with Enhanced Physiological Relevance.Advanced materials technologies · 2023Article
- The Use of Sensors in Blood-Brain Barrier-on-a-Chip Devices: Current Practice and Future Directions.Biosensors · 2023Review
- Gut-on-a-chip models for dissecting the gut microbiology and physiology.APL bioengineering · 2023Review
- Organ-On-A-Chip Models of the Blood-Brain Barrier: Recent Advances and Future Prospects.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- Contributions of the microbiome to intestinal inflammation in a gut-on-a-chip.Nano convergence · 2022Article
- Integrating mechanical sensor readouts into organ-on-a-chip platforms.Frontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transepithelial/transendothelial electrical resistance (TEER) measurements can be applied in organ-on-chips (OoCs) to estimate the barrier properties of a tissue or cell layer in a continuous, non-invasive, and label-free manner. Assessing the barrier integrity in in vitro models is valuable for studying and developing barrier targeting drugs. Several systems for measuring the TEER have been shown, but each of them having their own drawbacks. This article presents a cleanroom-free fabrication method for the integration of platinum electrodes in a polydimethylsiloxane OoC, allowing the real-time assessment of the barrier function by employing impedance spectroscopy. The proposed method and electrode arrangement allow visual inspection of the cells cultured in the device at the site of the electrodes, and multiplexing of both the electrodes in one OoC and the number of OoCs in one device. The effectiveness of our system is demonstrated by lining the OoC with intestinal epithelial cells, creating a gut-on-chip, where we monitored the formation, as well as the disruption and recovery of the cell barrier during a 21 day culture period. The application is further expanded by creating a blood-brain-barrier, to show that the proposed fabrication method can be applied to monitor the barrier formation in the OoC for different types of biological barriers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.