Evidence map›Paper›PMID 33859193›Full record

ArticleNature communications2021

Democratising deep learning for microscopy with ZeroCostDL4Mic.

Lucas von Chamier, Romain F Laine, Johanna Jukkala, Christoph Spahn, Daniel Krentzel, Elias Nehme, Martina Lerche, Sara Hernández-Pérez, Pieta K Mattila, Eleni Karinou and 12 more

Open access · goldAbstract read
In one paragraph

Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 233 papers.

0numbers the graph read from it
0cells of the map it votes in
233citing papers in PubMed
106.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

233 citing papers in PubMed, 544 citations in OpenAlex.

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  11. Heparan sulfate is essential for Drosophila FGF export.bioRxiv : the preprint server for biology · 2026
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173 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors at 11 institutions in 8 countries.

Lucas von Chamier *MRC-Laboratory for Molecular Cell Biology, University College London, London, UK.
Romain F Laine *MRC-Laboratory for Molecular Cell Biology, University College London, London, UK.
Johanna JukkalaTurku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland.
Christoph SpahnInstitute of Physical and Theoretical Chemistry, Goethe-University Frankfurt, Frankfurt, Germany.ORCID http://orcid.org/0000-0001-9886-2263
Daniel KrentzelElectron Microscopy Science Technology Platform, The Francis Crick Institute, London, UK.ORCID http://orcid.org/0000-0002-6234-7259
Elias NehmeDepartment of Electrical Engineering, Technion-Israel Institute of Technology, Haifa, Israel.
Martina LercheTurku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland.
Sara Hernández-PérezTurku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland.ORCID http://orcid.org/0000-0002-0227-1045
Pieta K MattilaTurku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland.ORCID http://orcid.org/0000-0003-2805-0686
Eleni KarinouCentre for Bacterial Cell Biology, Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle, UK.ORCID http://orcid.org/0000-0002-1099-4964
Séamus HoldenCentre for Bacterial Cell Biology, Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle, UK.ORCID http://orcid.org/0000-0002-7169-907X
Ahmet Can SolakChan Zuckerberg Biohub, San Francisco, CA, USA.
Alexander KrullCenter for Systems Biology Dresden (CSBD), Dresden, Germany.ORCID http://orcid.org/0000-0002-7778-7169
Tim-Oliver BuchholzCenter for Systems Biology Dresden (CSBD), Dresden, Germany.
Martin L JonesElectron Microscopy Science Technology Platform, The Francis Crick Institute, London, UK.ORCID http://orcid.org/0000-0003-0994-5652
Loïc A RoyerChan Zuckerberg Biohub, San Francisco, CA, USA.ORCID http://orcid.org/0000-0002-9991-9724
Christophe LeterrierAix Marseille Université, CNRS, INP UMR7051, NeuroCyto, Marseille, France.ORCID http://orcid.org/0000-0002-2957-2032
Yoav ShechtmanDepartment of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel.ORCID http://orcid.org/0000-0001-8498-5203
Florian JugCenter for Systems Biology Dresden (CSBD), Dresden, Germany.ORCID http://orcid.org/0000-0002-8499-5812
Mike HeilemannInstitute of Physical and Theoretical Chemistry, Goethe-University Frankfurt, Frankfurt, Germany.
Guillaume JacquemetTurku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland. guillaume.jacquemet@abo.fi.ORCID http://orcid.org/0000-0002-9286-920X
Ricardo HenriquesMRC-Laboratory for Molecular Cell Biology, University College London, London, UK. rjhenriques@igc.gulbenkian.pt.ORCID http://orcid.org/0000-0002-2043-5234
Åbo Akademi University · FIThe Francis Crick Institute · GBChan Zuckerberg Initiative (United States) · USGoethe University Frankfurt · DENewcastle University · GBTechnion – Israel Institute of Technology · ILCenter for Systems Biology Dresden · DECentre National de la Recherche Scientifique · FRHuman Technopole · ITMax Planck Institute for Physics · DEMRC Laboratory for Molecular Cell Biology · GB

Funding

Cancer Research UK FC001999Medical Research Council FC001999Medical Research Council MC_UU_00012/1Medical Research Council MR/K015826/1Medical Research Council MR/T027924/1Wellcome Trust 203276/Z/16/ZWellcome Trust 206670/Z/17/ZWellcome Trust FC001999
6 · The paper itself

Abstract

Deep Learning (DL) methods are powerful analytical tools for microscopy and can outperform conventional image processing pipelines. Despite the enthusiasm and innovations fuelled by DL technology, the need to access powerful and compatible resources to train DL networks leads to an accessibility barrier that novice users often find difficult to overcome. Here, we present ZeroCostDL4Mic, an entry-level platform simplifying DL access by leveraging the free, cloud-based computational resources of Google Colab. ZeroCostDL4Mic allows researchers with no coding expertise to train and apply key DL networks to perform tasks including segmentation (using U-Net and StarDist), object detection (using YOLOv2), denoising (using CARE and Noise2Void), super-resolution microscopy (using Deep-STORM), and image-to-image translation (using Label-free prediction - fnet, pix2pix and CycleGAN). Importantly, we provide suitable quantitative tools for each network to evaluate model performance, allowing model optimisation. We demonstrate the application of the platform to study multiple biological processes.

Indexed as

Deep LearningAnimalsCell Line, TumorCloud ComputingDatasets as TopicHumansImage Processing, Computer-AssistedMicroscopyPrimary Cell CultureRatsSoftware

Identifiers

PMID33859193
PMCPMC8050272
OpenAlexW3156859314

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.