Evidence map›Paper›PMID 33857754›Full record

ArticleBiosensors & bioelectronics2021

Development of flexible electrochemical impedance spectroscopy-based biosensing platform for rapid screening of SARS-CoV-2 inhibitors.

Lik-Voon Kiew, Chia-Yu Chang, Sheng-Yu Huang, Pei-Wen Wang, Choon-Han Heh, Chung-Te Liu, Chia-Hsin Cheng, Yi-Xiang Lu, Yen-Chen Chen, Yi-Xuan Huang and 17 more

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Article in Biosensors & bioelectronics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
4.3field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 61 citations in OpenAlex.

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  7. One-step synthesis of triethanolamine-capped Pt nanoparticle for colorimetric and electrochemiluminescent immunoassay of SARS-CoV spike proteins.Microchemical journal : devoted to the application of microtechniques in all branches of science · 2023
    Article
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  9. Nature-Inspired Surface Structures Design for Antimicrobial Applications.International journal of molecular sciences · 2023
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  19. Molecular Virology of SARS-CoV-2 and Related Coronaviruses.Microbiology and molecular biology reviews : MMBR · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

27 authors at 9 institutions in 2 countries.

Lik-Voon KiewDepartment of Pharmacology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia; Department of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan.
Chia-Yu ChangDepartment of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan; Center for Intelligent Drug Systems and Smart Bio-devices (IDS(2)B), National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan.
Sheng-Yu HuangResearch Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Laboratory Medicine, Linkuo Chang Gung Memorial Hospital, Taoyuan, 33301, Taiwan.
Pei-Wen WangInstitute of Oral Medicine and Department of Stomatology, College of Medicine, National Cheng Kung University Hospital, National Cheng Kung University, Tainan, 70101, Taiwan; Center of Applied Nanomedicine, National Cheng Kung University, Tainan, 70101, Taiwan.
Choon-Han HehDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Chung-Te LiuDivision of Nephrology, Department of Internal Medicine, Wan-Fang Hospital, Taipei Medical University, Taipei, 11696, Taiwan; Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, 11031, Taiwan.
Chia-Hsin ChengDepartment of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan.
Yi-Xiang LuDepartment of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan.
Yen-Chen ChenDepartment of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan.
Yi-Xuan HuangDepartment of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan.
Sheng-Yun ChangResearch Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Laboratory Medicine, Linkuo Chang Gung Memorial Hospital, Taoyuan, 33301, Taiwan; Bachelor Program in Artificial Intelligence, Chang Gung University, Taoyuan, 33301, Taiwan.
Huei-Yu TsaiInstitute of Oral Medicine and Department of Stomatology, College of Medicine, National Cheng Kung University Hospital, National Cheng Kung University, Tainan, 70101, Taiwan.
Yu-An KungResearch Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Laboratory Medicine, Linkuo Chang Gung Memorial Hospital, Taoyuan, 33301, Taiwan.
Peng-Nien HuangResearch Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Laboratory Medicine, Linkuo Chang Gung Memorial Hospital, Taoyuan, 33301, Taiwan.
Ming-Hua HsuDepartment of Chemistry, National Changhua University of Education, Changhua, 50007, Taiwan.
Bey-Fen LeoFaculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Yiing-Yee FooDepartment of Pharmacology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Chien-Hao SuDepartment of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan.
Kuo-Chen HsuPd Biomedical Co., Ltd., Taoyuan, 32560, Taiwan.
Po-Hsun HuangInstitute of Clinical Medicine, Cardiovascular Research Center, National Yang Ming Chiao Tung University, Taipei, 11121, Taiwan; Division of Cardiology, Taipei Veterans General Hospital, Taipei, 11217, Taiwan.
Chirk-Jenn NgDepartment of Primary Care Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Adeeba KamarulzamanDepartment of Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Chiun-Jye YuanDepartment of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan; Institute of Molecular Medicine and Bioengineering, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan.
Dar-Bin ShiehInstitute of Oral Medicine and Department of Stomatology, College of Medicine, National Cheng Kung University Hospital, National Cheng Kung University, Tainan, 70101, Taiwan; Center of Applied Nanomedicine, National Cheng Kung University, Tainan, 70101, Taiwan; Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan, 70101, Taiwan.
Shin-Ru ShihResearch Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Laboratory Medicine, Linkuo Chang Gung Memorial Hospital, Taoyuan, 33301, Taiwan. Electronic address: srshih@mail.cgu.edu.tw.
Lip-Yong ChungDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Malaya, 50603, Kuala Lumpur, Malaysia. Electronic address: chungly@um.edu.my.
Chia-Ching ChangDepartment of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan; Center for Intelligent Drug Systems and Smart Bio-devices (IDS(2)B), National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan; Department of Electrophysics, National Yang Ming Chiao Tung University, Hsinchu, 30010, Taiwan; Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan. Electronic address: ccchang01@faculty.nctu.edu.tw.
National Yang Ming Chiao Tung University · TWChang Gung University · TWUniversity of Malaya · MYChang Gung Memorial Hospital · TWTaipei Medical University · TWNational Changhua University of Education · TWNational Cheng Kung University · TWNational Cheng Kung University Hospital · TWTaipei Veterans General Hospital · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters the cells through the binding of its spike protein (S-protein) to the cell surface-expressing angiotensin-converting enzyme 2 (ACE2). Thus, inhibition of S-protein-ACE2 binding may impede SARS-CoV-2 cell entry and attenuate the progression of Coronavirus disease 2019 (COVID-19). In this study, an electrochemical impedance spectroscopy-based biosensing platform consisting of a recombinant ACE2-coated palladium nano-thin-film electrode as the core sensing element was fabricated for the screening of potential inhibitors against S-protein-ACE2 binding. The platform could detect interference of small analytes against S-protein-ACE2 binding at low analyte concentration and small volume (0.1 μg/mL and ~1 μL, estimated total analyte consumption < 4 pg) within 21 min. Thus, a few potential inhibitors of S-protein-ACE2 binding were identified. This includes (2S,3aS,6aS)-1-((S)-N-((S)-1-Carboxy-3-phenylpropyl)alanyl)tetrahydrocyclopenta[b] pyrrole-2-carboxylic acid (ramiprilat) and (2S,3aS,7aS)-1-[(2S)-2-[[(2S)-1-Carboxybutyl]amino]propanoyl]-2,3,3a,4,5,6,7,7a-octahydroindole-2-carboxylic acid (perindoprilat) that reduced the binding affinity of S-protein to ACE2 by 72% and 67%; and SARS-CoV-2 in vitro infectivity to the ACE2-expressing human oral cavity squamous carcinoma cells (OEC-M1) by 36.4 and 20.1%, respectively, compared to the PBS control. These findings demonstrated the usefulness of the developed biosensing platform for the rapid screening of modulators for S-protein-ACE2 binding.

Indexed as

Biosensing TechniquesCOVID-19Dielectric SpectroscopyHumansProtein BindingSARS-CoV-2Spike Glycoprotein, CoronavirusSpike Glycoprotein, CoronavirusACE2-SARS CoV 2 S-Protein interactionBiosensorElectrochemical impedance spectroscopy (EIS)Palladium nano-thin-film electrodeSARS-CoV-2 infection inhibitors

Identifiers

PMID33857754
PMCPMC8018905
OpenAlexW3148934558

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.