ArticlePloS one2021
Nutrigenomic analyses reveal miRNAs and mRNAs affected by feed restriction in the mammary gland of midlactation dairy cows.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 13 citations in OpenAlex.
- Differential expression of microRNAs in the prefrontal cortex as indicators of sheep welfare.BMC veterinary research · 2026Article
- Analysis of miRNAs in milk of four livestock species.BMC genomics · 2024Article
- MiRNome variations in milk fractions during feed restrictions of different intensities in dairy cows.BMC genomics · 2023Article
- Polymorphism at theAnimals : an open access journal from MDPI · 2023Article
- How Does Nutrition Affect the Epigenetic Changes in Dairy Cows?Animals : an open access journal from MDPI · 2023Review
- Milk proteins as a feed restriction signature indicating the metabolic adaptation of dairy cows.Scientific reports · 2022Article
- The Role of microRNAs in the Mammary Gland Development, Health, and Function of Cattle, Goats, and Sheep.Non-coding RNA · 2021Review
- Effect of feed restriction on dairy cow milk production: a review.Journal of animal science · 2021Review
- Nutritional Regulation of Mammary Gland Development and Milk Synthesis in Animal Models and Dairy Species.Genes · 2021Review
- Identification of Bovine miRNAs with the Potential to Affect Human Gene Expression.Frontiers in genetics · 2021Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objective of this study was to investigate the effects of feed restriction on mammary miRNAs and coding gene expression in midlactation cows. Five Holstein cows and 6 Montbéliarde cows underwent 6 days of feed restriction, during which feed allowance was reduced to meet 50% of their net energy for lactation requirements. Mammary biopsies were performed before and at the end of the restriction period. Mammary miRNA and mRNA analyses were performed using high-throughput sequencing and microarray analyses, respectively. Feed restriction induced a negative energy balance and decreased milk production and fat and protein yields in both breeds. Feed restriction modified the expression of 27 miRNAs and 374 mRNAs in mammary glands from Holstein cows, whereas no significant miRNA change was observed in Montbéliarde cows. Among the 27 differentially expressed miRNAs, 8 miRNAs were associated with dairy QTL. Analysis of target genes indicate that the 8 most abundantly expressed miRNAs control transcripts related to lipid metabolism, mammary remodeling and stress response. A comparison between the mRNAs targeted by the 8 most strongly expressed miRNAs and 374 differentially expressed mRNAs identified 59 mRNAs in common. The bioinformatic analyses of these 59 mRNAs revealed their implication in lipid metabolism and endothelial cell proliferation. These effects of feed restriction on mammary miRNAs and mRNAs observed in Holstein cows suggest a potential role of miRNAs in mammary structure and lipid biosynthesis that could explain changes in milk production and composition.
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