ArticlePloS one2021
Development and validation of a cellular host response test as an early diagnostic for sepsis.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Assessment of a cellular host response test to risk-stratify suspected COVID-19 patients in the Emergency Department setting.PloS one · 2022Trial
- Recent advances in screening, diagnosis, prognosis and personalized treatment of sepsis.European journal of microbiology & immunology · 2026Review
- Computational methods for inertial microfluidics: recent advances and future perspectives.Microsystems & nanoengineering · 2025Review
- Temporal trends of a cellular host response test for sepsis and a comparison with selected biomarkers of inflammation and infection.Scientific reports · 2025Article
- A Pilot Study Assessing the Utility of Quantitative Myeloid-Derived Suppressor Cell Measurements in Detecting Posttraumatic Infection.Critical care explorations · 2025Observational
- Evaluation of 3D-Printed Microfluidic Structures for Use in AML-Specific Biomarker Detection of PML::RARA.International journal of molecular sciences · 2025Article
- Harnessing the host response for precision infectious disease diagnosis.Clinical microbiology reviews · 2024Review
- Validation of a Novel, Rapid Sepsis Diagnostic for Emergency Department Use.Critical care explorations · 2024Article
- Quantifying both viscoelasticity and surface tension: Why sharp tips overestimate cell stiffness.Biophysical journal · 2024Article
- Variability in Provider Assessment of Sepsis and Potential of Host Response Technology to Address this Dilemma-Results of an Online Delphi Study.Journal of personalized medicine · 2023Article
- Rapid deformability cytometry for tissue biopsies.Nature biomedical engineering · 2023Article
- Rapid single-cell physical phenotyping of mechanically dissociated tissue biopsies.Nature biomedical engineering · 2023Article
- Costs and Consequences of a Novel Emergency Department Sepsis Diagnostic Test: The IntelliSep Index.Critical care explorations · 2023Article
- Rapid identification of sepsis in the emergency department.Journal of the American College of Emergency Physicians open · 2023Article
- Biophysical Changes of Leukocyte Activation (and NETosis) in the Cellular Host Response to Sepsis.Diagnostics (Basel, Switzerland) · 2023Article
- Numerical investigation of the dynamics of a rigid spherical particle in a vortical cross-slot flow at moderate inertia.Microsystems & nanoengineering · 2023Article
- Sepsis biomarkers and diagnostic tools with a focus on machine learning.EBioMedicine · 2022Review
- Reciprocity of Cell Mechanics with Extracellular Stimuli: Emerging Opportunities for Translational Medicine.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- Distinct timing of neutrophil spreading and stiffening during phagocytosis.Biophysical journal · 2022Article
- Assessment of a Cellular Host Response Test as a Sepsis Diagnostic for Those With Suspected Infection in the Emergency Department.Critical care explorations · 2021Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sepsis must be diagnosed quickly to avoid morbidity and mortality. However, the clinical manifestations of sepsis are highly variable and emergency department (ED) clinicians often must make rapid, impactful decisions before laboratory results are known. We previously developed a technique that allows the measurement of the biophysical properties of white blood cells as they are stretched through a microfluidic channel. In this study we describe and validate the resultant output as a model and score-the IntelliSep Index (ISI)-that aids in the diagnosis of sepsis in patients with suspected or confirmed infection from a single blood draw performed at the time of ED presentation. By applying this technique to a high acuity cohort with a 23.5% sepsis incidence (n = 307), we defined specific metrics-the aspect ratio and visco-elastic inertial response-that are more sensitive than cell size or cell count in predicting disease severity. The final model was trained and cross-validated on the high acuity cohort, and the performance and generalizability of the model was evaluated on a separate low acuity cohort with a 6.4% sepsis incidence (n = 94) and healthy donors (n = 72). For easier clinical interpretation, the ISI is divided into three interpretation bands of Green, Yellow, and Red that correspond to increasing disease severity. The ISI agreed with the diagnosis established by retrospective physician adjudication, and accurately identified subjects with severe illness as measured by SOFA, APACHE-II, hospital-free days, and intensive care unit admission. Measured using routinely collected blood samples, with a short run-time and no requirement for patient or laboratory information, the ISI is well suited to aid ED clinicians in rapidly diagnosing sepsis.
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