Evidence map›Paper›PMID 33855109›Full record

ArticleBio-protocol2021

An Image-based Dynamic High-throughput Analysis of Adherent Cell Migration.

Meng Sun, Bence Rethi, Akilan Krishnamurthy, Vijay Joshua, Heidi Wähämaa, Sergiu-Bogdan Catrina, Anca Catrina

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Meng SunRheumatology Unit, Department of Medicine, Karolinska Insititutet, Stockholm, Sweden.
Bence RethiRheumatology Unit, Department of Medicine, Karolinska Insititutet, Stockholm, Sweden.
Akilan KrishnamurthyRheumatology Unit, Department of Medicine, Karolinska Insititutet, Stockholm, Sweden.
Vijay JoshuaRheumatology Unit, Department of Medicine, Karolinska Insititutet, Stockholm, Sweden.
Heidi WähämaaRheumatology Unit, Department of Medicine, Karolinska Insititutet, Stockholm, Sweden.
Sergiu-Bogdan CatrinaDepartment of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden.
Anca CatrinaRheumatology Unit, Department of Medicine, Karolinska University Hospital Center for Rheumatology, Academic Specialist Centre, Stockholm, Sweden.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this protocol, we describe a method to monitor cell migration by live-cell imaging of adherent cells. Scratching assay is a common method to investigate cell migration or wound healing capacity. However, achieving homogenous scratching, finding the optimal time window for end-point analysis and performing an objective image analysis imply, even for practiced and adept experimenters, a high chance for variability and limited reproducibility. Therefore, our protocol implemented the assessment for cell mobility by using homogenous wound making, sequential imaging and automated image analysis. Cells were cultured in 96-well plates, and after attachment, homogeneous linear scratches were made using the IncuCyte

Indexed as

Cell migrationHigh-throughputLive cell imageScratching assayTime-lapse imaging

Identifiers

PMID33855109
PMCPMC8032497

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.