Evidence map›Paper›PMID 33854707›Full record

ArticleJournal of dental sciences2021

2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside-stimulated dental pulp stem cells-derived conditioned medium enhances cell activity and anti-inflammation.

Yu-Tang Chin, Che-Ming Liu, Ting-Yi Chen, Yao-Yu Chung, Chi-Yu Lin, Chao-Nan Hsiung, Yun-Shen Jan, Hsien-Chung Chiu, Earl Fu, Sheng-Yang Lee

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PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of dental sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 1 country.

Yu-Tang ChinSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Che-Ming LiuSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Ting-Yi ChenSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Yao-Yu ChungSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Chi-Yu LinSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Chao-Nan HsiungSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Yun-Shen JanGraduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan.
Hsien-Chung ChiuDepartment of Periodontology, School of Dentistry, National Defense Medical Center and Tri-Service General Hospital, Taipei, Taiwan.
Earl FuDepartment of Dentistry, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Xindian, New Taipei City, Taiwan.
Sheng-Yang LeeSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Stem Cell Technology (Taiwan) · TWTaipei Medical University · TWWan Fang Hospital · TWTaipei Tzu Chi Hospital · TWTri-Service General Hospital · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

background/purposeDental pulp stem cells (DPSCs) contribute to the regeneration of various tissues and have superior proliferation, immune privilege, and anti-inflammation properties to other mesenchymal stem cells. 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) not only enhances the aforementioned properties of DPSCs but also promotes self-renewal and reprogramming-like ability. However, whether THSG enhances the aforementioned properties and abilities through direct or indirect interaction mechanisms remains unclear. To address this knowledge gap, we examined the effects of THSG-stimulated DPSC-derived conditioned medium (THSG-CM) on the activity and anti-inflammation properties of cells. MATERIALS AND

methodsDPSCs were treated with various concentrations of THSG to produce THSG-CM, which was then collected, analyzed, and lyophilized. A cytokine profiling antibody assay was used to compare protein components between THSG-treated and nontreated CM. Human skin fibroblasts (HSFs) and human gingival fibroblasts (HGFs) were used to investigate the effect of THSG-CM on cell proliferation, anti-inflammation, and wound healing abilities; for this investigation, MTS assay, quantitative real-time PCR analysis, and 2-well silicone inserts wound model were conducted.

resultsWe observed that THSG enhanced the secretion of growth- and immune-associated proteins in THSG-CM and increased the proliferation of HSFs and HGFs. Furthermore, THSG-CM significantly attenuated lipopolysaccharide-stimulated mRNA levels of cytokines in both cells and improved wound healing abilities.

conclusionWe conclude that THSG-CM had more beneficial effects on cell activity and anti-inflammation in the HSFs and HGFs than DPSC-derived CM. DPSC-derived CM can be developed into a cell-free regenerative strategy in the future, and its therapeutic efficacy may be improved by THSG-CM.

Indexed as

2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucosideAnti-inflammationConditioned mediumDental pulp stem cellProliferationWound healing

Identifiers

PMID33854707
PMCPMC8025232
OpenAlexW3102087426

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.