ArticleVeterinary research2021
Evaluation using latent class models of the diagnostic performances of three ELISA tests commercialized for the serological diagnosis of Coxiella burnetii infection in domestic ruminants.
Article in Veterinary research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Systematic review ofOne health (Amsterdam, Netherlands) · 2026Review
- Surveillance forOne health (Amsterdam, Netherlands) · 2026Article
- Evaluation of two point-of-care molecular diagnostic platforms for rapid detection of equine Hendra virus.Veterinary and animal science · 2026Article
- [Long-term follow-up of a Coxiellosis outbreak in a goat flock].Tierarztliche Praxis. Ausgabe G, Grosstiere/Nutztiere · 2026Article
- B-cell peptide epitopes as diagnostic targets for Q fever in sheep.Frontiers in microbiology · 2026Article
- Impact of whole-herd vaccination on a caprine coxiellosis outbreak: a longitudinal study ofFrontiers in microbiology · 2026Article
- Evaluation of Three Recombinant Antigens for the Detection of Anti-Coxiella Antibodies in Cattle.Antibodies (Basel, Switzerland) · 2025Article
- Comparison of in-house immunofluorescence assay (IFA) and commercial enzyme-linked immunosorbent assay (ELISA) for Coxiella burnetii infection in cattle.Veterinary research communications · 2025Article
- Combination of serology and PCR analysis of environmental samples to assessApplied and environmental microbiology · 2025Article
- Nationwide Seroprevalence ofVeterinary sciences · 2025Article
- Detection of abortifacient agents in domestic ruminants, with a specific focus onOpen research Europe · 2025Article
- Seroepidemiology ofAnimals : an open access journal from MDPI · 2024Article
- Diagnostic serology test comparison for Q fever and Rift Valley fever in humans and livestock from pastoral communities.PLoS neglected tropical diseases · 2024Article
- Efficiency of recombinant Ybgf in a double antigen-ELISA for the detection of Coxiella antibodies in ruminants.Veterinary and animal science · 2024Article
- Efficacy and Safety of an Inactivated Phase IAnimals : an open access journal from MDPI · 2024Review
- Seroprevalence and renal carriage of pathogenic Leptospira in livestock in Cotonou, Benin.Veterinary medicine and science · 2024Article
- Review
- Ultraviolet C inactivation of Coxiella burnetii for production of a structurally preserved whole cell vaccine antigen.BMC microbiology · 2024Article
- Prevalence, Risk Factors, and Relationship between Reproductive Performance and the Presence of Antibodies against Coxiellosis in Dairy Farm Milk Tanks in the Northwest of Spain.Animals : an open access journal from MDPI · 2024Article
- A review of coxiellosis (Q fever) and brucellosis in goats and humans: Implications for disease control in smallholder farming systems in Southeast Asia.One health (Amsterdam, Netherlands) · 2023Review
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ELISA methods are the diagnostic tools recommended for the serological diagnosis of Coxiella burnetii infection in ruminants but their respective diagnostic performances are difficult to assess because of the absence of a gold standard. This study focused on three commercial ELISA tests with the following objectives (1) assess their sensitivity and specificity in sheep, goats and cattle, (2) assess the between- and within-herd seroprevalence distribution in these species, accounting for diagnostic errors, and (3) estimate optimal sample sizes considering sensitivity and specificity at herd level. We comparatively tested 1413 cattle, 1474 goat and 1432 sheep serum samples collected in France. We analyzed the cross-classified test results with a hierarchical zero-inflated beta-binomial latent class model considering each herd as a population and conditional dependence as a fixed effect. Potential biases and coverage probabilities of the model were assessed by simulation. Conditional dependence for truly seropositive animals was high in all species for two of the three ELISA methods. Specificity estimates were high, ranging from 94.8% [92.1; 97.8] to 99.2% [98.5; 99.7], whereas sensitivity estimates were generally low, ranging from 39.3 [30.7; 47.0] to 90.5% [83.3; 93.8]. Between- and within-herd seroprevalence estimates varied greatly among geographic areas and herds. Overall, goats showed higher within-herd seroprevalence levels than sheep and cattle. The optimal sample size maximizing both herd sensitivity and herd specificity varied from 3 to at least 20 animals depending on the test and ruminant species. This study provides better interpretation of three widely used commercial ELISA tests and will make it possible to optimize their implementation in future studies. The methodology developed may likewise be applied to other human or animal diseases.
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