Evidence map›Paper›PMID 33851500›Full record

ArticleChembiochem : a European journal of chemical biology2021

Expanding the Substrate Scope of Nitrating Cytochrome P450 TxtE by Active Site Engineering of a Reductase Fusion.

Rakesh Saroay, Gheorghe-Doru Roiban, Lona M Alkhalaf, Gregory L Challis

Abstract read
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Rakesh SaroayDepartment of Chemistry, University of Warwick, Coventry, CV4 7AL, UK.
Gheorghe-Doru RoibanSynthetic Biochemistry, Medicinal Science & Technology, GlaxoSmithKline, Medicines Research Centre, Gunnels Wood Road, Stevenage, SG1 2NY, UK.
Lona M AlkhalafDepartment of Chemistry, University of Warwick, Coventry, CV4 7AL, UK.
Gregory L ChallisDepartment of Chemistry, University of Warwick, Coventry, CV4 7AL, UK.ORCID 0000-0001-5976-3545

Funding

Biotechnology and Biological Sciences Research Council BB/K002341/1
6 · The paper itself

Abstract

Aromatic nitration reactions are a cornerstone of organic chemistry, but are challenging to scale due to corrosive reagents and elevated temperatures. The cytochrome P450 TxtE nitrates the indole 4-position of l-tryptophan at room temperature using NO, O

Indexed as

Binding SitesCytochrome P-450 Enzyme SystemMolecular StructureNitratesOxidation-ReductionSubstrate SpecificityTryptophanCytochrome P-450 Enzyme SystemNitratesTryptophanBiocatalysisIndoleNitrationProtein Engineering

Identifiers

PMID33851500
PMCPMC8359946

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.