Evidence map›Paper›PMID 33851184›Full record

ArticlemedRxiv : the preprint server for health sciences2021

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.

Maria Jose Lista, Pedro M Matos, Thomas J A Maguire, Kate Poulton, Elena Ortiz-Zapater, Robert Page, Helin Sertkaya, Ana M Ortega-Prieto, Aoife M O'Byrne, Clement Bouton and 26 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in medRxiv : the preprint server for health sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

36 authors.

Maria Jose ListaKing's College London Diagnostics Team at Guy's Campus (London, UK).
Pedro M MatosKing's College London Diagnostics Team at Guy's Campus (London, UK).
Thomas J A MaguireKing's College London Diagnostics Team at Guy's Campus (London, UK).
Kate PoultonKing's College London Diagnostics Team at Guy's Campus (London, UK).
Elena Ortiz-ZapaterKing's College London Diagnostics Team at Guy's Campus (London, UK).
Robert PageKing's College London Diagnostics Team at Guy's Campus (London, UK).
Helin SertkayaKing's College London Diagnostics Team at Guy's Campus (London, UK).
Ana M Ortega-PrietoDept. Infectious Diseases, School of Immunology and Microbial Sciences. Guy's Campus, King's College London SE1 9RT, UK.
Aoife M O'ByrneKing's College London Diagnostics Team at Guy's Campus (London, UK).
Clement BoutonKing's College London Diagnostics Team at Guy's Campus (London, UK).
Ruth E DickensonKing's College London Diagnostics Team at Guy's Campus (London, UK).
Mattia FicarelliKing's College London Diagnostics Team at Guy's Campus (London, UK).
Jose M Jimenez-GuardeñoDept. Infectious Diseases, School of Immunology and Microbial Sciences. Guy's Campus, King's College London SE1 9RT, UK.
Mark HowardKing's College London Diagnostics Team at Guy's Campus (London, UK).
Gilberto BetancorKing's College London Diagnostics Team at Guy's Campus (London, UK).
Rui Pedro GalaoKing's College London Diagnostics Team at Guy's Campus (London, UK).
Suzanne PickeringKing's College London Diagnostics Team at Guy's Campus (London, UK).
Adrian W SignellKing's College London Diagnostics Team at Guy's Campus (London, UK).
Harry WilsonKing's College London Diagnostics Team at Guy's Campus (London, UK).
Penelope CliffViapath pathology laboratories at St Thomas' Hospital.
Mark Tan Kia IkCentre for Infectious Diseases Research, St Thomas' Hospital (London, UK).
Amita PatelCentre for Infectious Diseases Research, St Thomas' Hospital (London, UK).
Eithne MacMahonCentre for Infectious Diseases Research, St Thomas' Hospital (London, UK).
Emma CunninghamCentre for Infectious Diseases Research, St Thomas' Hospital (London, UK).
Katie DooresKing's College London Diagnostics Team at Guy's Campus (London, UK).
Monica AgromayorKing's College London Diagnostics Team at Guy's Campus (London, UK).
Juan Martin-SerranoKing's College London Diagnostics Team at Guy's Campus (London, UK).
Esperanza PeruchaKing's College London Diagnostics Team at Guy's Campus (London, UK).
Hannah E MischoKing's College London Diagnostics Team at Guy's Campus (London, UK).
Manu Shankar-HariKing's College London Diagnostics Team at Guy's Campus (London, UK).
Rahul BatraCentre for Infectious Diseases Research, St Thomas' Hospital (London, UK).
Jonathan EdgeworthCentre for Infectious Diseases Research, St Thomas' Hospital (London, UK).
Mark ZuckermanKing's College London Diagnostics Team at Guy's Campus (London, UK).
Michael H MalimKing's College London Diagnostics Team at Guy's Campus (London, UK).
Stuart NeilKing's College London Diagnostics Team at Guy's Campus (London, UK).
Rocio Teresa Martinez-NunezKing's College London Diagnostics Team at Guy's Campus (London, UK).

Funding

X-ray Crystallographic Fragment Screening CoreU54AI150472 · NIAID · SEATTLE CHILDREN'S HOSPITAL · PI ARNOLD, EDWARD · 2019 to 2021
$15.7M
Ultrapotent Inhibitors of Wild-type and Multi-drug Resistant HIVR01AI076119 · NIAID · UNIVERSITY OF MISSOURI-COLUMBIA · PI SARAFIANOS, STEFAN G · 2008 to 2018
$4.1M
Medical Research Council MR/S009191/1Medical Research Council MR/S023747/1NIAID NIH HHS R01 AI076119NIAID NIH HHS U54 AI150472Wellcome Trust
6 · The paper itself

Abstract

There is a worldwide need for reagents to perform SARS-CoV-2 detection. Some laboratories have implemented kit-free protocols, but many others do not have the capacity to develop these and/or perform manual processing. We provide multiple workflows for SARS-CoV-2 nucleic acid detection in clinical samples by comparing several commercially available RNA extraction methods: QIAamp Viral RNA Mini Kit (QIAgen), RNAdvance Blood/Viral (Beckman) and Mag-Bind Viral DNA/RNA 96 Kit (Omega Bio-tek). We also compared One-step RT-qPCR reagents: TaqMan Fast Virus 1-Step Master Mix (FastVirus, ThermoFisher Scientific), qPCRBIO Probe 1-Step Go Lo-ROX (PCR Biosystems) and Luna

Identifiers

PMID33851184
PMCPMC8043481

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.