ArticleSTAR protocols2021
Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer.
Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- Elevated nitric oxide during colitis restrains GM-CSF production in ILC3 cells via suppressing an AhR-Cyp4f13-NF-κB axis.Nature communications · 2025Article
- A new era in melanoma immunotherapy: focus on DCs metabolic reprogramming.Cancer cell international · 2025Review
- Tolerogenic dendritic cell-mediated regulatory T cell differentiation by Chinese herbal formulation attenuates colitis progression.Journal of advanced research · 2025Article
- Spermidine suppresses DC activation via eIF5A hypusination and metabolic adaptation.Discovery immunology · 2025Article
- Live-cell metabolic analysis of oligodendroglia isolated from postnatal mouse brain and spinal cord.STAR protocols · 2022Article
- Isolation of human and mouse myeloid-derived suppressor cells for metabolic analysis.STAR protocols · 2022Article
- Protocol for the assessment of human T cell activation by real-time metabolic flux analysis.STAR protocols · 2022Article
- Measuring CPT-1-mediated respiration in permeabilized cells and isolated mitochondria.STAR protocols · 2021Article
- Attenuating Effect ofFrontiers in nutrition · 2021Article
- Mitochondrial Reactive Oxygen Species Are Essential for the Development of Psoriatic Inflammation.Frontiers in immunology · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dendritic cell (DC) maturation induced by Toll-like receptor (TLR) agonists requires the activation of downstream metabolic changes. Here, we provide a detailed protocol to measure glycolysis, mitochondrial respiration, and fatty acid oxidation in mouse bone-marrow-derived DCs with the Seahorse XF24 extracellular flux (XF) analyzer. XF analysis with the Seahorse bioanalyzer has become a standard method to measure bioenergetic functions in cells, and this protocol can be adapted to other immune cells. For complete information on using this protocol, please refer to Gotoh et al. (2018).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.