ArticleScientific reports2021
The efficacy of CRISPR-mediated cytosine base editing with the RPS5a promoter in Arabidopsis thaliana.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Chloroplast- and mitochondrion-specific random C-to-T mutagenesis for forward genetics of organelle genomes.The Plant journal : for cell and molecular biology · 2026Article
- Establishing efficient multi-gene editing tools for papaya.Horticulture research · 2026Article
- Development of herbicide-resistant peanut via prime editing and optimization of editing efficiency.Plant physiology · 2026Article
- Identification of optimal adenine and cytosine base editors for genome editing in Arabidopsis and soybean.BMB reports · 2025Article
- Precise genome editing process and its applications in plants driven by AI.Functional & integrative genomics · 2025Review
- CRISPR-based gene editing in plants: Focus on reagents and their delivery tools.BioImpacts : BI · 2025Review
- TALE-based C-to-T base editor for multiple homologous genes with flexible precision.Plant biotechnology (Tokyo, Japan) · 2024Article
- An efficient multiplex approach to CRISPR/Cas9 gene editing in citrus.Plant methods · 2024Article
- Multiplexed gene editing in citrus by using a multi-intron containing Cas9 gene.Transgenic research · 2024Article
- Developing a highly efficient CGBE base editor in watermelon.Horticulture research · 2023Article
- Virus-Induced Gene Silencing (VIGS): A Powerful Tool for Crop Improvement and Its Advancement towards Epigenetics.International journal of molecular sciences · 2023Review
- Cytosine base editors optimized for genome editing in potato protoplasts.Frontiers in genome editing · 2023Article
- Genome editing.Scientific reports · 2022Article
- Heritable base-editing in Arabidopsis using RNA viral vectors.Plant physiology · 2022Article
- From Genome Sequencing to CRISPR-Based Genome Editing for Climate-Resilient Forest Trees.International journal of molecular sciences · 2022Review
- Comprehending the evolution of gene editing platforms for crop trait improvement.Frontiers in genetics · 2022Review
- Current status and trends in forest genomics.Forestry research · 2022Review
- The Functional Association ofInternational journal of molecular sciences · 2021Article
- Employing CRISPR/Cas Technology for the Improvement of Potato and Other Tuber Crops.Frontiers in plant science · 2021Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR/Cas9-mediated genome editing is an important and versatile technology in modern biological research. Recent advancements include base-editing CRISPR tools that enable targeted nucleotide substitutions using a fusion protein comprising a nickase variant of Cas9 and a base deaminase. Improvements in base editing efficiencies and inheritable of edited loci need to be made to make CRISPR a viable system in plants. Here, we report efficiency of cytosine base editors (CBEs) in Arabidopsis thaliana by applying the strong endogenous RPS5a promoter to drive the expression of nickase Cas9 and either rAPOBEC1 from rat (BE3) or the PmCDA1 activation-induced cytidine deaminase from sea lamprey (AIDv2). Compared with the strong heterologous CaMV35S promoter of viral origin, the RPS5a promoter improved CBE efficiency by 32% points with the number of T
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