ArticleScientific reports2021
Autofluorescence enhancement for label-free imaging of myelinated fibers in mammalian brains.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- Advances in multiscale myelin imaging: from classical histology to functional insights.Frontiers in cellular neuroscience · 2026Review
- Myelinated fiber labeling and orientation mapping of the human brain with light-sheet fluorescence microscopy.NeuroImage · 2025Article
- Micron-resolution fiber mapping in histology independent of sample preparation.Nature communications · 2025Article
- Quantitative cytoarchitectural phenotyping of deparaffinized human brain tissues.Communications biology · 2025Article
- Anisotropic light propagation in human brain white matter.Neurophotonics · 2025Article
- Micron-resolution fiber mapping in histology independent of sample preparation.bioRxiv : the preprint server for biology · 2025Article
- Historical trends in neuroanatomical tract-tracing techniques.Anatomical science international · 2025Review
- An Improved SHANEL Procedure for Clearing and Staining Brain Tissue from Multiple Species.International journal of molecular sciences · 2025Article
- Automated detection of c-Fos-expressing neurons using inhomogeneous background subtraction in fluorescent images.Neurobiology of learning and memory · 2025Article
- Toward a brighter constellation: multiorgan neuroimaging of neural and vascular dynamics in the spinal cord and brain.Neurophotonics · 2024Article
- Practical considerations for birefringence microscopy of myelin structure: Microscope design and tissue processing for effective imaging.Imaging neuroscience (Cambridge, Mass.) · 2024Article
- Towards a Brighter Constellation: Multi-Organ Neuroimaging of Neural and Vascular Dynamics in the Spinal Cord and Brain.bioRxiv : the preprint server for biology · 2023Article
- Multiscale label-free imaging of myelin in human brain tissue with polarization-sensitive optical coherence tomography and birefringence microscopy.Biomedical optics express · 2023Article
- A cellular resolution atlas of Broca's area.Science advances · 2023Article
- Using light and X-ray scattering to untangle complex neuronal orientations and validate diffusion MRI.eLife · 2023Article
- Sex, strain, and lateral differences in brain cytoarchitecture across a large mouse population.eLife · 2023Article
- Fiber enhancement and 3D orientation analysis in label-free two-photon fluorescence microscopy.Scientific reports · 2023Article
- Article
- Simultaneous Probing of Metabolism and Oxygenation of Tumors In Vivo Using FLIM of NAD(P)H and PLIM of a New Polymeric Ir(III) Oxygen Sensor.International journal of molecular sciences · 2022Article
- Automated computation of nerve fibre inclinations from 3D polarised light imaging measurements of brain tissue.Scientific reports · 2022Article
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
Analyzing the structure of neuronal fibers with single axon resolution in large volumes is a challenge in connectomics. Different technologies try to address this goal; however, they are limited either by the ineffective labeling of the fibers or in the achievable resolution. The possibility of discriminating between different adjacent myelinated axons gives the opportunity of providing more information about the fiber composition and architecture within a specific area. Here, we propose MAGIC (Myelin Autofluorescence imaging by Glycerol Induced Contrast enhancement), a tissue preparation method to perform label-free fluorescence imaging of myelinated fibers that is user friendly and easy to handle. We exploit the high axial and radial resolution of two-photon fluorescence microscopy (TPFM) optical sectioning to decipher the mixture of various fiber orientations within the sample of interest. We demonstrate its broad applicability by performing mesoscopic reconstruction at a sub-micron resolution of mouse, rat, monkey, and human brain samples and by quantifying the different fiber organization in control and Reeler mouse's hippocampal sections. Our study provides a novel method for 3D label-free imaging of nerve fibers in fixed samples at high resolution, below micrometer level, that overcomes the limitation related to the myelinated axons exogenous labeling, improving the possibility of analyzing brain connectivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.