ArticleNature communications2021
Direct 1,3-butadiene biosynthesis in Escherichia coli via a tailored ferulic acid decarboxylase mutant.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 50 citations in OpenAlex.
- Balancing Dimerization and Hydrogenation Kinetics by Stabilizing CuAngewandte Chemie (International ed. in English) · 2026Article
- NHC-Mediated Radical Acylation Catalyzed by Thiamine- and Flavin-Dependent Enzymes.Journal of the American Chemical Society · 2025Article
- Genetically encoded biosensors for the circular plastics bioeconomy.Metabolic engineering communications · 2024Article
- Design and construction of artificial metabolic pathways for the bioproduction of useful compounds.Plant biotechnology (Tokyo, Japan) · 2024Article
- Engineering a coenzyme-independent dioxygenase for one-step production of vanillin from ferulic acid.Applied and environmental microbiology · 2024Article
- Styrene Production in Genetically EngineeredBiotech (Basel (Switzerland)) · 2024Article
- Microbial production ofSynthetic and systems biotechnology · 2023Article
- Transcriptome profiling of Paraburkholderia aromaticivorans AR20-38 during ferulic acid bioconversion.AMB Express · 2022Article
- In Silico Design Strategies for the Production of Target Chemical Compounds Using Iterative Single-Level Linear Programming Problems.Biomolecules · 2022Article
- Toolbox for the structure-guided evolution of ferulic acid decarboxylase (FDC).Scientific reports · 2022Article
- Irreversible and Self-Healing Electrically Conductive Hydrogels Made of Bio-Based Polymers.International journal of molecular sciences · 2022Review
- Directed evolution of prenylated FMN-dependent Fdc supports efficient in vivo isobutene production.Nature communications · 2021Article
- Synthetic Enzyme-Catalyzed COChemSusChem · 2021Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The C4 unsaturated compound 1,3-butadiene is an important monomer in synthetic rubber and engineering plastic production. However, microorganisms cannot directly produce 1,3-butadiene when glucose is used as a renewable carbon source via biological processes. In this study, we construct an artificial metabolic pathway for 1,3-butadiene production from glucose in Escherichia coli by combining the cis,cis-muconic acid (ccMA)-producing pathway together with tailored ferulic acid decarboxylase mutations. The rational design of the substrate-binding site of the enzyme by computational simulations improves ccMA decarboxylation and thus 1,3-butadiene production. We find that changing dissolved oxygen (DO) levels and controlling the pH are important factors for 1,3-butadiene production. Using DO-stat fed-batch fermentation, we produce 2.13 ± 0.17 g L
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