Evidence map›Paper›PMID 33850140›Full record

ArticleNature communications2021

B1a and B2 cells are characterized by distinct CpG modification states at DNMT3A-maintained enhancers.

Vinay S Mahajan, Hamid Mattoo, Na Sun, Vinayak Viswanadham, Grace J Yuen, Hugues Allard-Chamard, Maimuna Ahmad, Samuel J H Murphy, Annaiah Cariappa, Yesim Tuncay and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.9field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 32 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. OMIP-111: Immune-Profiling of T Helper 1 (Th1), Th2, and Th17 Signatures in Murine Splenocytes by Targeting Intracellular Cytokines.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2025
    Article
  5. Review
  6. Review
  7. Methylation of T and B Lymphocytes in Autoimmune Rheumatic Diseases.Clinical reviews in allergy & immunology · 2024
    Review
  8. Review
  9. Review
  10. Article
  11. Review
  12. Lupus, DNA Methylation, and Air Pollution: A Malicious Triad.International journal of environmental research and public health · 2022
    Review
  13. Review
  14. Radiation therapy induces immunosenescence mediated by p90RSK.Frontiers in cardiovascular medicine · 2022
    Article
  15. Review
  16. Activation ofCancer research · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Vinay S Mahajan *Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.ORCID 0000-0003-0814-2989
Hamid Mattoo *Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
Na Sun *Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
Vinayak Viswanadham *Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.ORCID 0000-0002-0236-6490
Grace J YuenRagon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
Hugues Allard-ChamardRagon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
Maimuna AhmadRagon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
Samuel J H MurphyRagon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.ORCID 0000-0002-5694-3572
Annaiah CariappaRagon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
Yesim TuncayRagon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
Shiv PillaiRagon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA. pillai@helix.mgh.harvard.edu.ORCID 0000-0002-9905-875X
Ragon Institute of MGH, MIT and Harvard · USBrigham and Women's Hospital · USHarvard University · US

Funding

Pilot & Feasibility ProgramP30DK043351 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI Ramnik J Xavier · 1991 to 2026
$35.3M
The importance of the IgG4 glycome in IgG4-related disease(s)U19AI110495 · NIAID · MASSACHUSETTS GENERAL HOSPITAL · PI SHIV Subramaniam PILLAI · 2014 to 2026
$7.9M
Pathogenesis of IgG4 related diseaseK08AI113163 · NIAID · BRIGHAM AND WOMEN'S HOSPITAL · PI MAHAJAN, VINAY SUBHASH · 2014 to 2018
$953k
NIAID NIH HHS K08 AI113163NIAID NIH HHS U19 AI110495NIDDK NIH HHS P30 DK043351
6 · The paper itself

Abstract

The B1 and B2 lineages of B cells contribute to protection from pathogens in distinct ways. The role of the DNA CpG methylome in specifying these two B-cell fates is still unclear. Here we profile the CpG modifications and transcriptomes of peritoneal B1a and follicular B2 cells, as well as their respective proB cell precursors in the fetal liver and adult bone marrow from wild-type and CD19-Cre Dnmt3a floxed mice lacking DNMT3A in the B lineage. We show that an underlying foundational CpG methylome is stably established during B lineage commitment and is overlaid with a DNMT3A-maintained dynamic methylome that is sculpted in distinct ways in B1a and B2 cells. This dynamic DNMT3A-maintained methylome is composed of novel enhancers that are closely linked to lineage-specific genes. While DNMT3A maintains the methylation state of these enhancers in both B1a and B2 cells, the dynamic methylome undergoes a prominent programmed demethylation event during B1a but not B2 cell development. We propose that the methylation pattern of DNMT3A-maintained enhancers is determined by the coincident recruitment of DNMT3A and TET enzymes, which regulate the developmental expression of B1a and B2 lineage-specific genes.

Indexed as

AnimalsB-LymphocytesCell DifferentiationCpG IslandsDNA (Cytosine-5-)-MethyltransferasesDNA MethylationDNA Methyltransferase 3AEpigenomeGene ExpressionMiceMice, KnockoutRegulatory Sequences, Nucleic AcidTranscriptomeDNA (Cytosine-5-)-MethyltransferasesDNA Methyltransferase 3ADnmt3a protein, mouse

Identifiers

PMID33850140
PMCPMC8044213
OpenAlexW3155128465

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.