Evidence map›Paper›PMID 33847974›Full record

ArticlePulmonary therapy2021

In Vitro Effects of 5-Lipoxygenase Pathway Inhibition on Rhinovirus-Associated Bronchial Epithelial Inflammation.

Irini Spyridaki, Styliani Taka, Chrysanthi Skevaki, Aikaterini Trochoutsou, Nikolaos G Papadopoulos

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pulmonary therapy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 3 countries.

Irini SpyridakiAllergy Department, 2nd Pediatric Clinic, National and Kapodistrian University of Athens, Athens, Greece.
Styliani TakaAllergy Department, 2nd Pediatric Clinic, National and Kapodistrian University of Athens, Athens, Greece. staka@med.uoa.gr.ORCID http://orcid.org/0000-0001-7607-8005
Chrysanthi SkevakiAllergy Department, 2nd Pediatric Clinic, National and Kapodistrian University of Athens, Athens, Greece.
Aikaterini TrochoutsouAllergy Department, 2nd Pediatric Clinic, National and Kapodistrian University of Athens, Athens, Greece.
Nikolaos G PapadopoulosAllergy Department, 2nd Pediatric Clinic, National and Kapodistrian University of Athens, Athens, Greece.
National and Kapodistrian University of Athens · GRPhilipps University of Marburg · DE

Funding

Deutsche Zentrum für Lungenforschung KFO 309 (P10)Rhön Klinikum, and the Deutsche Forschungsgemeinschaft (DFG) SK 317/1-1 AOBJ: 661487Uniklinikum Giessen und Marburg SFB 1021 (C04)
6 · The paper itself

Abstract

introductionThe leukotriene pathway may be implicated in the induction of virus-induced inflammation. Respiratory epithelial cells may express low levels of 5-lipoxygenase (5-LO) and release leukotrienes (LTs) C4, D4, and E4, upon exposure to viruses or other stimuli. Enhanced expression of 5-LO pathway proteins after rhinovirus (RV) infection has previously been described. We hypothesized that anti-leukotriene treatment of epithelial cells, with or without exposure to RV-infected peripheral blood mononuclear cells (PBMCs)-conditioned media, may inhibit RV-induced up-regulation of inflammatory cytokines.

methodsPBMCs from a healthy donor were exposed to RV1B and supernatants were harvested at 48 h post infection. BEAS-2B cells were infected with RV, with or without conditioning with the PBMC supernatant. Treatment with anti-LT agents was performed either on both PBMCs and BEAS-2B or at the bronchial epithelial level only, with varying concentrations of montelukast (CysLT receptor antagonist) or MK-886 [FLAP(5-lipoxygenase-activating-protein) inhibitor]. Evaluation of the inflammatory cytokines IL-8, RANTES, IL-11, IL-6, and IP-10 was performed using ELISA.

resultsOur results show that anti-LT treatment of RV-infected bronchial epithelial cells suppresses epithelial RV-mediated cytokine production, independent of conditioning.

conclusionsThis observation may represent an indirect mode of action of the anti-leukotrienes in virus-induced asthma.

Indexed as

5-lipoxygenaseAnti-inflammatoryLeukotrienesRhinovirus

Identifiers

PMID33847974
PMCPMC8137792
OpenAlexW3155782845

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.