Expression, Purification, and Solution-State NMR Analysis of the Two Human Single-Stranded DNA-Binding Proteins hSSB1 (NABP2/OBFC2B) and hSSB2 (NAPB1/OBFC2A).

Serene El-Kamand, Mar-Dean Du Plessis, Teegan Lawson, Liza Cubeddu, Roland Gamsjaeger

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Serene El-Kamand *School of Science, Western Sydney University, Penrith, NSW, Australia.

Mar-Dean Du Plessis *School of Science, Western Sydney University, Penrith, NSW, Australia.

Teegan Lawson *School of Science, Western Sydney University, Penrith, NSW, Australia.

Liza CubedduSchool of Science, Western Sydney University, Penrith, NSW, Australia. l.cubeddu@westernsydney.edu.au.

Roland GamsjaegerSchool of Science, Western Sydney University, Penrith, NSW, Australia. r.gamsjaeger@westernsydney.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single-stranded DNA-binding proteins (SSBs) are essential to all living organisms as protectors and guardians of the genome. Apart from the well-characterized RPA, humans have also evolved two further SSBs, termed hSSB1 and hSSB2. Over the last few years, we have used NMR spectroscopy to determine the molecular and structural details of both hSSBs and their interactions with DNA and RNA. Here we provide a detailed overview of how to express and purify recombinant versions of these important human proteins for the purpose of detailed structural analysis by high-resolution solution-state NMR.

Indexed as

Cloning, MolecularDNA, BacterialDNA-Binding ProteinsEscherichia coliFermentationHumansIsotope LabelingMagnetic Resonance SpectroscopyMitochondrial ProteinsProtein BindingRecombinant ProteinsRNA, BacterialDNA, BacterialDNA-Binding ProteinsMitochondrial ProteinsNABP2 protein, humanRecombinant ProteinsRNA, BacterialSSBP2 protein, humanCancerDNA repairhSSB1hSSB2NMR

Identifiers

PMID33847962

DOI10.1038/srep27446

What OpenQuestion holds

Textmetadata

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.