Evidence map›Paper›PMID 33847954›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Magnetic Tweezers-Based Single-Molecule Assays to Study Interaction of E. coli SSB with DNA and RecQ Helicase.

Debjani Bagchi, Weiting Zhang, Samar Hodeib, Bertrand Ducos, Vincent Croquette, Maria Manosas

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.0field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 6 institutions in 3 countries.

Debjani BagchiPhysics Department, Faculty of Science, Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, India.
Weiting ZhangLaboratoire de Physique Statistique, Département de physique de l'ENS, École normale supérieure, PSL Research University, Université Paris Diderot, Sorbonne Paris Cité, Sorbonne Universités, UPMC University Paris 06, CNRS, Paris, France.
Samar HodeibHigh Throughput qPCR Core Facility of the ENS, qPCR-HD-GPC, Université PSL, CNRS, IBENS, 46 rue d'Ulm, Paris, France.
Bertrand DucosLaboratoire de Physique Statistique, Département de physique de l'ENS, École normale supérieure, PSL Research University, Université Paris Diderot, Sorbonne Paris Cité, Sorbonne Universités, UPMC University Paris 06, CNRS, Paris, France.
Vincent CroquetteLaboratoire de Physique Statistique, Département de physique de l'ENS, École normale supérieure, PSL Research University, Université Paris Diderot, Sorbonne Paris Cité, Sorbonne Universités, UPMC University Paris 06, CNRS, Paris, France.
Maria ManosasSmall Biosystems Lab, Departament de Física de la Matèria Condensada, Facultat de Física, Universitat de Barcelona, Barcelona, Spain. mmanosas@gmail.com.
École Normale Supérieure - PSL · FRInstitut de Biologie de l'École Normale Supérieure · FRMaharaja Sayajirao University of Baroda · INSorbonne Université · FRUniversitat de Barcelona · ESUniversité Paris Sciences et Lettres · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ability of magnetic tweezers to apply forces and measure molecular displacements has resulted in its extensive use to study the activity of enzymes involved in various aspects of nucleic acid metabolism. These studies have led to the discovery of key aspects of protein-protein and protein-nucleic acid interaction, uncovering dynamic heterogeneities that are lost to ensemble averaging in bulk experiments. The versatility of magnetic tweezers lies in the possibility and ease of tracking multiple parallel single-molecule events to yield statistically relevant single-molecule data. Moreover, they allow tracking both fast millisecond dynamics and slow processes (spanning several hours). In this chapter, we present the protocols used to study the interaction between E. coli SSB, single-stranded DNA (ssDNA), and E. coli RecQ helicase using magnetic tweezers. In particular, we propose constant force and force modulation assays to investigate SSB binding to DNA, as well as to characterize various facets of RecQ helicase activity stimulation by SSB.

Indexed as

DNA, BacterialDNA-Binding ProteinsEscherichia coliEscherichia coli ProteinsMagnetic PhenomenaProtein BindingRecQ HelicasesSingle Molecule ImagingTime FactorsDNA, BacterialDNA-Binding ProteinsEscherichia coli ProteinsRecQ HelicasesSSB protein, E coliDNAForceMagnetic tweezersRecQ helicaseSingle moleculeSSB

Identifiers

PMID33847954
OpenAlexW3156839272

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.