Evidence map›Paper›PMID 33847953›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

In Vivo Binding of Single-Stranded DNA-Binding Protein to Stalled Replication Fork Helicases.

Cong Yu, Piero R Bianco

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Cong YuDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, Omaha, Nebraska, USA.
Piero R BiancoDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, Omaha, Nebraska, USA. pbianco2014@gmail.com.

Funding

Mechanistic studies of stalled DNA replication fork rescueR01GM100156 · NIGMS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI BIANCO, PIERO R, LYUBCHENKO, YURI L · 2013 to 2021
$4.0M
Insight into the mechanism of action of the SSB interactomeR01GM144414 · NIGMS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI LYUBCHENKO, YURI L · 2022 to 2025
$1.5M
NIGMS NIH HHS R01 GM100156NIGMS NIH HHS R01 GM144414
6 · The paper itself

Abstract

Understanding protein-protein interactions is key to unraveling protein function in vivo. Here we describe a dual/triple-plasmid system that enables co-expression of two, or three, recombinant proteins harboring different affinity tags in the same Escherichia coli cell. This novel protein expression system provides a platform to understand protein-protein interactions and enables researchers to study protein complex formation and in vivo localization.

Indexed as

Binding SitesDNA, BacterialDNA-Binding ProteinsDNA HelicasesDNA ReplicationDNA, Single-StrandedElectrophoresis, Polyacrylamide GelEscherichia coliEscherichia coli ProteinsPlasmidsProtein BindingRecombinant ProteinsDNA, BacterialDNA-Binding ProteinsDNA HelicasesDNA, Single-StrandedEscherichia coli ProteinspriA protein, E coliRecG protein, E coliRecombinant ProteinsDNA helicasePriARecGSingle-strand binding proteinSSBStalled fork rescue

Identifiers

PMID33847953
PMCPMC9476560

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.