Evidence map›Paper›PMID 33847947›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Purification of Human Complement Component C4 and Sample Preparation for Structural Biology Applications.

Alessandra Zarantonello, Sofia Mortensen, Nick S Laursen, Gregers R Andersen

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Alessandra ZarantonelloDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
Sofia MortensenDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
Nick S LaursenDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
Gregers R AndersenDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark. gra@mbg.au.dk.
Aarhus University · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Activated complement component C4 (C4b) is the nonenzymatic component of the classical pathway (CP) convertases of the complement system. Preparation of C4 and C4b samples suitable for structural biology studies is challenging due to low yields and complexity of recombinant C4 production protocols reported so far and heterogeneity of C4 in native sources. Here we present a purification protocol for human C4 and describe sample preparation methods for structural investigation of C4 and its complexes by crystallography, small angle X-ray scattering, and electron microscopy.

Indexed as

BiologyChromatography, GelComplement C4Crystallography, X-RayHumansIon ExchangeMicroscopy, ElectronNegative StainingProtein ConformationProteomicsScattering, Small AngleX-Ray DiffractionComplement C4Complement C4Gel filtrationIon exchangeNegative stain electron microscopySmall angle X-ray scatteringX-ray crystallography

Identifiers

PMID33847947
OpenAlexW3155789413

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.