ArticleArchives of toxicology2021
Mechanisms of deoxynivalenol-induced endocytosis and degradation of tight junction proteins in jejunal IPEC-J2 cells involve selective activation of the MAPK pathways.
Article in Archives of toxicology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 63 citations in OpenAlex.
- Fusobacterium nucleatum-Derived Outer Membrane Vesicles Disrupt Epithelial Barrier in Oral Lichen Planus via JNK/c-JUN Mediated Claudin-4 Downregulation.International dental journal · 2026Article
- The Combination of Chinese Medicine Monomers Reduces 7S Globulin-Induced Damage to IPEC-J2 Cells In Vitro by Modulating NF-κB/MAPK Pathway-Related Markers.Veterinary sciences · 2026Article
- Dynamic cellular heterogeneity revealed through a time-resolved single-cell atlas: assessment of porcine intestinal organoids as an in vitro model for deoxynivalenol and zearalenone.Journal of animal science and biotechnology · 2026Article
- Targeting Mycotoxin Toxicity: From Molecular Mechanisms to Nutritional Interventions.Veterinary sciences · 2026Review
- Effect of Pre-Exposure to Deoxynivalenol on the Response of Porcine Intestinal Epithelial Cells to F18Toxins · 2026Article
- Serum Uric Acid-Reducing Effect and Intestinal Mucosal Barrier-Repairing Function ofMicroorganisms · 2026Article
- Residual based anomaly detection framework for variant caller dispatch in DNA sequencing data.Frontiers in microbiology · 2026Article
- Mycotoxins and the Intestinal Epithelium: From Barrier Injury to Stem Cell Dysfunction.Toxins · 2025Review
- Deoxynivalenol and pigs: review of harmful effect of Mycotoxin on swine health.Porcine health management · 2025Review
- Review
- Zinc Oxide Administration Relieves the Diarrhea of ETEC K88-Infected Piglets by Reducing Ileal Apoptosis and Maintaining Gut Microbial Balance.Veterinary sciences · 2025Article
- Interplay between nutrition, microbiota, and immunity in rotavirus infection: insights from human and animal models.Frontiers in veterinary science · 2025Review
- Circadian rhythm in mycotoxin-induced immunotoxicity: an emerging regulatory axis?Frontiers in pharmacology · 2025Review
- Methionine and its derivatives in dairy cow nutrition: implications for intestinal barrier function, periparturient performance, and metabolic health.Frontiers in veterinary science · 2025Review
- Investigation of Deoxynivalenol Contamination in Local Area and Evaluation of Its Multiple Intestinal Toxicity.Toxins · 2024Article
- The Alleviating Effect of Taxifolin on Deoxynivalenol-Induced Damage in Porcine Intestinal Epithelial Cells.Veterinary sciences · 2024Article
- Selenomethionine Alleviates Deoxynivalenol-Induced Oxidative Injury in Porcine Intestinal Epithelial Cells Independent of MAPK Pathway Regulation.Antioxidants (Basel, Switzerland) · 2024Article
- Protective Effects of Carbonated Chitosan Montmorillonite on Vomitoxin-Induced Intestinal Inflammation.Polymers · 2024Article
- Selenomethionine Supplementation Mitigates Liver Dysfunction, Oxidative Injury and Apoptosis through Enhancing Antioxidant Capacity and Inhibiting JNK MAPK Pathway in Piglets Fed Deoxynivalenol-Contaminated Diets.Antioxidants (Basel, Switzerland) · 2024Article
- Olive Mill Waste-Water Extract Enriched in Hydroxytyrosol and Tyrosol Modulates Host-Pathogen Interaction in IPEC-J2 Cells.Animals : an open access journal from MDPI · 2024Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycotoxin contamination in foods is a major risk factor for human and animal health due to its prevalence in cereals and their by-products. Deoxynivalenol (DON), mainly produced by Fusarium genera, is the most common mycotoxin detected in cereal products. Deoxynivalenol disrupts intestinal barrier function and decreases protein levels of tight junction proteins (TJP). However, the overall mechanism by which DON regulates specific TJP turnover and epithelial cell integrity remains unclear. Herein, we show that DON (2 μM) decreases the protein stability and accelerates the degradation of TJP in the lysosome. Interestingly, pretreatment of cells with dynasore (a dynamin-dependent endocytosis inhibitor) protected against DON-induced degradation of claudin-3 and 4. Immunofluorescence analysis also shows that the decreased membrane presence of claudin-4 and ZO-1 induced by DON is reversible with dynamin inhibition, whereas the pretreatment with cytochalasin D (an actin-dependent endocytosis inhibitor) reverses the degradation of claudin-1 and 4 induced by DON. We also show that the endocytosis and degradation of claudin-1 is regulated by p38 mitogen-activated protein kinase (MAPK), whereas the endocytosis of claudin-4 and ZO-1 is mediated by c-Jun-N-terminal kinase (JNK). Resveratrol, with JNK inhibitory activity, also prevents the endocytosis and degradation of claudin-4 and ZO-1 and protects against DON-induced decrease in transepithelial electrical resistance (TEER) and increase in FITC-dextran permeability. Collectively, this study, for the first time, shows that DON accelerates the endocytosis and degradation of TJP and this is regulated by the activation of p38 MAPK and JNK signaling pathways. Therefore, natural bioactive compounds with p38 MAPK and JNK inhibitory activities may be effective in preventing the DON-induced TJP disruption and preserve gut barrier function in vivo.
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