ArticleJournal of applied microbiology2021
Comparison of the culture method with multiplex PCR for the confirmation of Legionella spp. and Legionella pneumophila.
Article in Journal of applied microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- A Hybrid Occupational Risk Assessment ofMicroorganisms · 2026Article
- Progress of Rapid Detection Technology for Aquatic Microorganisms: A Comprehensive Review.Microorganisms · 2026Review
- How to identify respiratory pathogens in primary health care - a review on the benefits, prospects and pitfalls in using point of care tests.Infection · 2025Review
- LegioTyper: Rapid typing of Legionella pneumophila serogroup 1 by flow-based chemiluminescence sandwich microarray immunoassay.Analytical and bioanalytical chemistry · 2025Article
- Two-stage surveillance ofSAGE open medicine · 2025Article
- From Tradition to Innovation: Diverse Molecular Techniques in the Fight Against Infectious Diseases.Diagnostics (Basel, Switzerland) · 2024Review
- Occurrence of pathogenicJournal of water and health · 2024Article
- Immunomagnetic separation coupled with flow cytometry for the analysis of Legionella pneumophila in aerosols.Analytical and bioanalytical chemistry · 2023Article
- Application of Multiplex Fluorescence Polymerase Chain Reaction for Detecting Pathogenic Bacteria in Sputum Samples from Patients with Lower Respiratory Tract Infection.Infection and drug resistance · 2023Article
- Detection of Pathogens and Antimicrobial Resistance Genes in Ventilator-Associated Pneumonia by Metagenomic Next-Generation Sequencing Approach.Infection and drug resistance · 2023Article
- Evaluation of a Most Probable Number Method for Detection and Quantification ofPathogens (Basel, Switzerland) · 2022Article
- Overview of the Clinical and Molecular Features of Legionella Pneumophila: Focus on Novel Surveillance and Diagnostic Strategies.Antibiotics (Basel, Switzerland) · 2022Review
- Comparison of the culture method with multiplex PCR for the confirmation of Legionella spp. and Legionella pneumophila.Journal of applied microbiology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsThe detection and enumeration of Legionella spp. in water samples are typically performed via a cultural technique standardized in ISO 11731. This method is time-consuming (up to 15 days), and the specificity of the confirmation step is questionable. This study proposes the use of multiplex polymerase chain reaction (PCR) to confirm presumptive Legionella colonies directly from the culture plate; this shortens the response time by 2-5 days while still reporting results in colony forming units (CFU). METHODS AND
resultsTwo laboratories analysed a total of 290 colonies to compare the confirmation step of Legionella spp. and Legionella pneumophila in accordance with ISO 11731 by culture growth and agglutination vs multiplex PCR. Discordant results were resolved by the swiss national reference laboratory. The data were evaluated following ISO 16140 and showed that the PCR-technique had higher specificity.
conclusionsThe confirmation of Legionella spp., L. pneumophila and L. pneumophila serogroup 1 by multiplex PCR allows detection of positive colonies more rapidly and with higher specificity. SIGNIFICANCE AND IMPACT OF THE STUDY: The study highlights a possibility to shorten the response time significantly during the enumeration of Legionella spp. and achieving a higher specificity while adhering to the legally recognized reporting in CFU.
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