Evidence map›Paper›PMID 33846784›Full record

ArticleInternational journal of molecular medicine2021

Knockdown of lncRNA MIR4435‑2HG and ST8SIA1 expression inhibits the proliferation, invasion and migration of prostate cancer cells

Pengyi Xing, Ye Wang, Li Zhang, Chao Ma, Jianping Lu

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
2.9field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 36 citations in OpenAlex.

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  11. Targeting PI3K/Akt signaling in prostate cancer therapy.Journal of cell communication and signaling · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Pengyi Xing *Department of Radiology, The First Affiliated Hospital of Naval Medical University, Shanghai 200433, P.R. China.
Ye Wang *Department of Urology, Chinese People's Liberation Army (PLA) General Hospital/PLA Medical School, Beijing 100853, P.R. China.
Li ZhangDepartment of Endocrinology, PLA Joint Logistics Support Force No. 989 Hospital, Luoyang, Henan 471600, P.R. China.
Chao MaDepartment of Radiology, The First Affiliated Hospital of Naval Medical University, Shanghai 200433, P.R. China.
Jianping LuDepartment of Radiology, The First Affiliated Hospital of Naval Medical University, Shanghai 200433, P.R. China.
Chinese People's Liberation Army · CNPeople's Liberation Army 401 Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Prostate cancer is a main health risk for males with a high incidence and mortality. The present study aimed to examine the effects of long non‑coding RNA (lncRNA) MIR4435‑2HG binding with ST8SIA1 on the proliferation, invasion and migration of prostate cancer cells via the activation of the FAK/AKT/β‑catenin signaling pathway. The expression of MIR4435‑2HG and ST8SIA1 in prostate cancer cell lines, and the transfection efficacy were analyzed by RT‑qPCR. The proliferation, clone formation ability, and the invasion and migration of transfected cells were detected by CCK‑8 assay, clone formation assay, Transwell assay and wound healing assay, respectively. Plasmids were injected subcutaneously into mice to construct a xenograft tumor model. The expression levels of proteins related to proliferation, apoptosis, invasion and migration, and the FAK/AKT/β‑catenin pathway were detected by western blot analysis. The results revealed that MIR4435‑2HG expression was increased in the prostate cancer cell lines and MIR4435‑2HG expression was the highest in the PC‑3 cells. Interference with MIR4435‑2HG inhibited the proliferation, clone formation ability, and the invasion and migration of PC‑3 cells, as well as tumor growth by suppressing the activation of the FAK/AKT/β‑catenin signaling pathway. MIR4435‑2HG was demonstrated to target ST8SIA1. ST8SIA1 expression was also increased in the prostate cancer cell lines and MIR4435‑2HG expression was the highest in the PC‑3 cells. Interference with ST8SIA1 inhibited the promoting effects of MIR4435‑2HG on the proliferation, invasion and migration of PC‑3 cells, as well as tumor growth by suppressing the activation of the FAK/AKT/β‑catenin signaling pathway. On the whole, the present study demonstrates that interference with MIR4435‑2HG, combined with ST8SIA1, inhibits the proliferation, invasion and migration of prostate cancer cells

Indexed as

Gene Expression Regulation, NeoplasticSignal TransductionAnimalsbeta CateninCell Line, TumorCell MovementCell ProliferationFocal Adhesion Kinase 1Gene Knockdown TechniquesHumansMaleMiceMice, NudePC-3 CellsProstatic NeoplasmsProto-Oncogene Proteins c-aktalpha-N-acetylneuraminate alpha-2,8-sialyltransferasebeta CateninFocal Adhesion Kinase 1Proto-Oncogene Proteins c-aktPTK2 protein, humanRNA, Long NoncodingSialyltransferasesFAK/AKT/β‑catenin signaling pathwaylncRNA MIR4435‑2HGprostate cancer cellsST8SIA1

Identifiers

PMID33846784
PMCPMC8041483
OpenAlexW3143391409

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.