ArticleCommunications biology2021
An engineered channelrhodopsin optimized for axon terminal activation and circuit mapping.
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 20 citations in OpenAlex.
- CAST/ELKS-endophilin-A interaction ensures synaptic vesicle pool size.The Journal of cell biology · 2026Article
- Hippocampal long-range GABAergic neurons target hippocampal-projecting cells of the supramammillary area.The Journal of physiology · 2026Article
- Functional interrogation of neuronal connections by chemoptogenetic presynaptic ablation.Science advances · 2026Article
- Ectopic action potentials in regular spiking neurons in the anesthetized mouse.Journal of neurophysiology · 2026Article
- Temporally Coordinated Activity among Motor Cortex, Thalamus, and Thalamic Reticular Nucleus Neurons during Rat Forelimb Movements.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Cancer neuroscience: signaling pathways and new therapeutic strategies for cancer.Signal transduction and targeted therapy · 2026Review
- Neural regulation of immune evasion in breast cancer: a multiscale neuro-immune framework.Frontiers in immunology · 2026Review
- Activation of silent synapses driven by emerging technologies: mechanisms, disease associations, and prospects for clinical translation.Frontiers in synaptic neuroscience · 2026Review
- Optogenetics and chemogenetics: key tools for modulating neural circuits in rodent models of depression.Frontiers in neural circuits · 2025Review
- Protein design accelerates the development and application of optogenetic tools.Computational and structural biotechnology journal · 2025Review
- Monosynaptic ventral tegmental area glutamate projections to the locus coeruleus enhance aversive processing.bioRxiv : the preprint server for biology · 2024Article
- Behavioral control through the direct, focal silencing of neuronal activity.Cell chemical biology · 2024Article
- All-optical presynaptic plasticity induction by photoactivated adenylyl cyclase targeted to axon terminals.Cell reports methods · 2024Article
- Beyond a Transmission Cable-New Technologies to Reveal the Richness in Axonal Electrophysiology.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Review
- Rat hippocampal CA1 region represents learning-related action and reward events with shorter latency than the lateral entorhinal cortex.Communications biology · 2023Article
- Parabrachial-to-parasubthalamic nucleus pathway mediates fear-induced suppression of feeding in male mice.Nature communications · 2022Article
- Exploiting the molecular diversity of the synapse to investigate neuronal communication: A guide through the current toolkit.The European journal of neuroscience · 2022Review
- Automated and parallelized spike collision tests to identify spike signal projections.iScience · 2022Article
- Optogenetics at the presynapse.Nature neuroscience · 2022Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optogenetic tools such as channelrhodopsin-2 (ChR2) enable the manipulation and mapping of neural circuits. However, ChR2 variants selectively transported down a neuron's long-range axonal projections for precise presynaptic activation remain lacking. As a result, ChR2 activation is often contaminated by the spurious activation of en passant fibers that compromise the accurate interpretation of functional effects. Here, we explored the engineering of a ChR2 variant specifically localized to presynaptic axon terminals. The metabotropic glutamate receptor 2 (mGluR2) C-terminal domain fused with a proteolytic motif and axon-targeting signal (mGluR2-PA tag) localized ChR2-YFP at axon terminals without disturbing normal transmission. mGluR2-PA-tagged ChR2 evoked transmitter release in distal projection areas enabling lower levels of photostimulation. Circuit connectivity mapping in vivo with the Spike Collision Test revealed that mGluR2-PA-tagged ChR2 is useful for identifying axonal projection with significant reduction in the polysynaptic excess noise. These results suggest that the mGluR2-PA tag helps actuate trafficking to the axon terminal, thereby providing abundant possibilities for optogenetic experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.