Evidence map›Paper›PMID 33846399›Full record

ArticleScientific reports2021

Prognostic value of intratumoral Fusobacterium nucleatum and association with immune-related gene expression in oral squamous cell carcinoma patients.

Cindy Neuzillet, Manon Marchais, Sophie Vacher, Marc Hilmi, Anne Schnitzler, Didier Meseure, Renaud Leclere, Charlotte Lecerf, Coraline Dubot, Emmanuelle Jeannot and 11 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers, 5 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
83citing papers in PubMed, 5 pooled it
11.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

83 citing papers in PubMed, 5 syntheses or guidelines pooled it, 113 citations in OpenAlex.

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  5. [Progress in the relationship between head and neck squamous cell carcinom and the microbial community].Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery · 2023
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  7. Functional Heterogeneity ofMicroorganisms · 2026
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23 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 3 institutions in 1 country.

Cindy NeuzilletMedical Oncology Department, Institut Curie, Versailles Saint-Quentin University, 35 rue Dailly, 92210, Saint-Cloud, France. cindy.neuzillet@curie.fr.
Manon MarchaisDepartment of Genetics, Institut Curie, PSL Research University, Paris, France.
Sophie VacherDepartment of Genetics, Institut Curie, PSL Research University, Paris, France.
Marc HilmiMedical Oncology Department, Institut Curie, Versailles Saint-Quentin University, 35 rue Dailly, 92210, Saint-Cloud, France.
Anne SchnitzlerDepartment of Genetics, Institut Curie, PSL Research University, Paris, France.
Didier MeseureDepartment of Pathology, Institut Curie, PSL Research University, Paris, France.
Renaud LeclereDepartment of Pathology, Institut Curie, PSL Research University, Paris, France.
Charlotte LecerfDepartment of Drug Development and Innovation (D3i), Institut Curie, Saint-Cloud, Paris, France.
Coraline DubotDepartment of Drug Development and Innovation (D3i), Institut Curie, Saint-Cloud, Paris, France.
Emmanuelle JeannotDepartment of Genetics, Institut Curie, PSL Research University, Paris, France.
Jerzy KlijanienkoDepartment of Pathology, Institut Curie, PSL Research University, Paris, France.
Odette MarianiDepartment of Pathology, Institut Curie, PSL Research University, Paris, France.
Valentin CalugaruDepartment of Radiotherapy, Institut Curie, PSL Research University, Paris, France.
Caroline HoffmannDepartment of Surgery, Institut Curie, PSL Research University, Paris, France.
Maria LesnikDepartment of Surgery, Institut Curie, PSL Research University, Paris, France.
Nathalie BadoisDepartment of Surgery, Institut Curie, PSL Research University, Paris, France.
Edith BorcomanDepartment of Drug Development and Innovation (D3i), Institut Curie, Saint-Cloud, Paris, France.
Eliane PiaggioINSERM U932 Research Unit, Institut Curie, PSL Research University, Paris, France.
Maud KamalDepartment of Drug Development and Innovation (D3i), Institut Curie, Saint-Cloud, Paris, France.
Christophe Le TourneauDepartment of Drug Development and Innovation (D3i), Institut Curie, Saint-Cloud, Paris, France.
Ivan BiecheDepartment of Genetics, Institut Curie, PSL Research University, Paris, France.
Institut Curie · FRUniversité Paris Sciences et Lettres · FRDélégation Paris 5 · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Changes in the oral microbiome, particularly Fusobacterium nucleatum, are associated with oral squamous cell carcinoma (OSCC). F. nucleatum has been reported to modulate local immunity in cancers. We aimed to assess the association between intratumoral F. nucleatum and clinico-pathological features, relapse, and overall survival (OS) in two independent cohorts of patients with OSCC, and to explore the interplay with immune-related genes. We retrospectively analyzed tissue samples from a first cohort of 122 patients with head and neck squamous cell carcinoma, including 61 OSCC (cohort #1), and a second cohort of 90 additional OSCC (cohort #2). We then performed a sensitivity analysis on the merged cohort of OSCC patients (N = 151). F. nucleatum 16S rRNA gene sequences were quantified using real-time quantitative PCR. The presence of gram-negative bacteria and macrophages was confirmed by LPS and CD163 immunostainings, respectively. F. nucleatum positivity was associated with older age, less alcohol and combined alcohol plus tobacco consumption, and less frequent lymph node invasion. There was a trend for a lower recurrence rate in F. nucleatum-positive cases, with less metastatic relapses compared to F. nucleatum-negative tumors, and significantly longer OS, relapse-free and metastasis-free survival. F. nucleatum status was independently associated with OS in multivariate analysis. Immune-related gene and immunohistochemistry analyses showed that gram-negative bacteria load inversely correlated with M2 macrophages. F. nucleatum-associated OSCC has a specific immune microenvironment, is more frequent in older, non-drinking patients, and associated with a favorable prognosis.

Indexed as

Gastrointestinal MicrobiomeHead and Neck NeoplasmsSquamous Cell Carcinoma of Head and NeckTumor MicroenvironmentFemaleFusobacterium nucleatumGene Expression Regulation, NeoplasticHumansMaleMiddle AgedRetrospective Studies

Identifiers

PMID33846399
PMCPMC8041800
OpenAlexW3153656616

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.