Trial reportAging2021
Potential reversal of epigenetic age using a diet and lifestyle intervention: a pilot randomized clinical trial.
Trial report in Aging, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. It is linked to 3 registered trials, which are not on this map. Cited by 215 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
The Impact of a Polyphenol-Rich Supplement on Epigenetic and Cellular Markers of Immune Age
Biological Effects in Consumers of Resveratrol-enriched Wine
Application of a Precision Lifestyle Medicine Clinical Program for a Sample of IFHAS Screening Cohort
Who cites it
215 citing papers in PubMed, 2 syntheses or guidelines pooled it, 335 citations in OpenAlex.
- An ELOVL2-Based Epigenetic Clock for Forensic Age Prediction: A Systematic Review.International journal of molecular sciences · 2023Pooled it
- Pooled it
- Trial
- Trial
- Nucleotides as an Anti-Aging Supplementation in Older Adults: A Randomized Controlled Trial (TALENTs study).Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Trial
- Trial
- Trial
- The CALERIE Genomic Data Resource.Nature aging · 2025Trial
- Long-term Impact of a 10-Year Intensive Lifestyle Intervention on a Deficit Accumulation Frailty Index: Action for Health in Diabetes Trial.The journals of gerontology. Series A, Biological sciences and medical sciences · 2023Trial
- The effect of polyphenols on DNA methylation-assessed biological age attenuation: the DIRECT PLUS randomized controlled trial.BMC medicine · 2023Trial
- High-Intensity interval training reduces transcriptomic age: A randomized controlled trial.Aging cell · 2023Trial
- Exercise and epigenetic ages in older adults with myeloid malignancies.European journal of medical research · 2023Trial
- Epigenetic age deceleration reflects exercise-induced cardiorespiratory fitness improvements.GeroScience · 2026Article
- Dietary preference, biological aging, and incident dementia: a prospective cohort study.GeroScience · 2026Article
- Article
- Review
- Chronic Pain in Older Adults: Risk Factors and Prevention.Life (Basel, Switzerland) · 2026Review
- Longitudinal associations of one-carbon metabolism biomarkers with biological and epigenetic aging in older adults in India.GeroScience · 2026Article
- Intake of ultra-processed foods and accelerated epigenetic aging of colorectal tissues.JNCI cancer spectrum · 2026Article
- A Mechanism-Based Framework for Anti-Aging Strategies: From Metabolic Regulation to Senotherapy and Stem Cell-Based Interventions.Aging cell · 2026Review
155 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Manipulations to slow biological aging and extend healthspan are of interest given the societal and healthcare costs of our aging population. Herein we report on a randomized controlled clinical trial conducted among 43 healthy adult males between the ages of 50-72. The 8-week treatment program included diet, sleep, exercise and relaxation guidance, and supplemental probiotics and phytonutrients. The control group received no intervention. Genome-wide DNA methylation analysis was conducted on saliva samples using the Illumina Methylation Epic Array and DNAmAge was calculated using the online Horvath DNAmAge clock (2013). The diet and lifestyle treatment was associated with a 3.23 years decrease in DNAmAge compared with controls (p=0.018). DNAmAge of those in the treatment group decreased by an average 1.96 years by the end of the program compared to the same individuals at the beginning with a strong trend towards significance (p=0.066). Changes in blood biomarkers were significant for mean serum 5-methyltetrahydrofolate (+15%, p=0.004) and mean triglycerides (-25%, p=0.009). To our knowledge, this is the first randomized controlled study to suggest that specific diet and lifestyle interventions may reverse Horvath DNAmAge (2013) epigenetic aging in healthy adult males. Larger-scale and longer duration clinical trials are needed to confirm these findings, as well as investigation in other human populations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.