Evidence map›Paper›PMID 33844293›Full record

ArticleJournal of microscopy2021

Elemental mapping of labelled biological specimens at intermediate energy loss in an energy-filtered TEM acquired using a direct detection device.

Ranjan Ramachandra, Mason R Mackey, Junru Hu, Steven T Peltier, Nguyen-Huu Xuong, Mark H Ellisman, Stephen R Adams

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of microscopy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Ranjan RamachandraDepartment of Neurosciences, University of California, San Diego, La Jolla, California, USA.ORCID 0000-0001-8603-7354
Mason R MackeyDepartment of Neurosciences, University of California, San Diego, La Jolla, California, USA.
Junru HuDepartment of Neurosciences, University of California, San Diego, La Jolla, California, USA.
Steven T PeltierDepartment of Neurosciences, University of California, San Diego, La Jolla, California, USA.
Nguyen-Huu XuongCenter for Research in Biological Systems, National Center for Microscopy and, Imaging Research, University of California, San Diego, La Jolla, California, USA.
Mark H EllismanDepartment of Neurosciences, University of California, San Diego, La Jolla, California, USA.
Stephen R AdamsDepartment of Pharmacology, University of California, San Diego, La Jolla, California, USA.
University of California San Diego · US

Funding

New Probe and Methods for Correlated LM & EMR01GM086197 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ADAMS, STEPHEN ROY, BOASSA, DANIELA · 2008 to 2020
$8.3M
National Center for Microscopy and Imaging Research: A BRAIN Technology Integration and Dissemination ResourceU24NS120055 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ELLISMAN, MARK H · 2021 to 2025
$5.4M
The National Center for Microscopy and Imaging Research, a Community-wide Scientific ResourceR24GM137200 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ELLISMAN, MARK H · 2020 to 2022
$3.0M
Advancing Multi-Color EM via Direct Detector-enabled 4D-STEMR01GM138780 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ELLISMAN, MARK H · 2020 to 2023
$1.7M
NIGMS NIH HHS R01 GM086197NIGMS NIH HHS R01 GM138780NIGMS NIH HHS R24 GM137200NINDS NIH HHS U24 NS120055
6 · The paper itself

Abstract

The technique of colour EM that was recently developed enabled localisation of specific macromolecules/proteins of interest by the targeted deposition of diaminobenzidine (DAB) conjugated to lanthanide chelates. By acquiring lanthanide elemental maps by energy-filtered transmission electron microscopy (EFTEM) and overlaying them in pseudo-colour over the conventional greyscale TEM image, a colour EM image is generated. This provides a powerful tool for visualising subcellular component/s, by the ability to clearly distinguish them from the general staining of the endogenous cellular material. Previously, the lanthanide elemental maps were acquired at the high-loss M

Indexed as

Lanthanoid Series ElementsMicroscopy, Energy-Filtering Transmission ElectronDiagnostic ImagingElectronsStaining and LabelingLanthanoid Series Elementscolour EMDABDDDdirect detection deviceEFTEMintermediate losslanthanide DABlow lossspectrum imaging

Identifiers

PMID33844293
PMCPMC8316382
OpenAlexW3153686174

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.