ArticleClinical oral investigations2021
Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods.
Article in Clinical oral investigations, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- A Comparison of Endodontic Microbiomes Associated With Symptomatic and Asymptomatic Apical Periodontitis by Next-Generation Sequencing.International endodontic journal · 2026Article
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- Antibacterial efficacy and molecular docking analysis ofJournal of conservative dentistry and endodontics · 2025Article
- Taxonomic Diversity and Clinical Correlations in Periapical Lesions by Next-Generation Sequencing Analysis.Genes · 2025Article
- The impact of passive ultrasonic irrigation on the bond strength of two different self-etch adhesives to human pulp chamber dentine: a laboratory investigation.BMC oral health · 2025Article
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- Comparative Efficacy of Sonic, Ultrasonic, and Diode Laser-Activated Irrigation with Combination of NaOCl/EDTA/CHX Solutions AgainstClinical, cosmetic and investigational dentistry · 2025Article
- Whole-genome amplification as a tool to improve bacterial detection by PCR in microbiological samples after endodontic treatment.Frontiers in oral health · 2025Article
- Guanylate-binding protein 5-mediated autophagy can promote the clearance of intracellular F. nucleatum in dental pulp cells during pulpitis.BMC oral health · 2024Article
- Antibiofilm efficacy of a calcium silicate-based intracanal medicament against Fusobacterium nucleatum strains.Scientific reports · 2024Article
- Pectin as a Biomaterial in Regenerative Endodontics-Assessing Biocompatibility and Antibacterial Efficacy against Common Endodontic Pathogens: An In Vitro Study.Bioengineering (Basel, Switzerland) · 2024Article
- Nanoarchitectonics-Based Materials as a Promising Strategy in the Treatment of Endodontic Infections.Pharmaceutics · 2024Review
- The effects of antimicrobial peptides buCaTHL4B and Im-4 on infectious root canal biofilms.Frontiers in bioengineering and biotechnology · 2024Article
- A new model ofHeliyon · 2023Article
- Microbiological profile of root canals indicated for endodontic retreatment due to secondary endodontic infections or for prosthetic reasons.Clinical oral investigations · 2023Article
- Antagonistic interaction between two key endodontic pathogensJournal of oral microbiology · 2023Article
- Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
objectiveFusobacterium nucleatum is an important oral pathogen involved in endodontic infections. This study aimed to assess the frequency of Fusobacterium nucleatum in primary and secondary endodontic infections and its associations with the clinical features in a Brazilian population by using both culture and nested PCR methods.
methodsA total of 100 microbial samples from patients with primary (n=50) and secondary endodontic infections (n=50) were analyzed by using culture and nested PCR methods. Strict anaerobic techniques were used for culture and identification of F. nucleatum. The DNA extracted from the samples was analyzed for the presence of target species by using species-specific primers.
resultsCulture and nested PCR methods detected F. nucleatum, respectively, in 11/100 and 82/100 root canals. F. nucleatum was isolated by culture from 10/50 (20%) root canals with primary infections and from 1/50 (2%) root canal with secondary/persistent infections. Nested PCR detected F. nucleatum in 42/50 (84%) root canals with primary infections and in 40/50 (80%) root canals with secondary/persistent endodontic infections. F. nucleatum was associated with spontaneous pain, tenderness to percussion, pain on palpation, swelling, tooth mobility, wet root canals, hemorrhagic exudate, tooth decay, inadequate restoration, and poor endodontic filling.
conclusionF. nucleatum was found in more cases of primary endodontic infections than in cases of secondary/persistent ones. A higher prevalence of F. nucleatum was detected by using the nested PCR method than by using culture. The presence of F. nucleatum in the root canals was associated with several clinical features. CLINICAL RELEVANCE: The high prevalence of F. nucleatum in the root canals detected by molecular methods, and its association with several clinical features reveals the importance of these species in the development of apical pathologies and reinforces the need of an endodontic treatment directed to bacterial elimination.
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