Evidence map›Paper›PMID 33843275›Full record

ArticleStem cells and development2021

Biologically Derived, Three-Dimensional, Embryonic Scaffolds for Long-Term Cardiomyocyte Culture.

Mary G Garry, Stefan Kren, Joseph B Wenger, Daniel J Garry

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells and development, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Mary G GarryLillehei Heart Institute (LHI), University of Minnesota, Minneapolis, Minnesota, USA.
Stefan KrenLillehei Heart Institute (LHI), University of Minnesota, Minneapolis, Minnesota, USA.
Joseph B WengerLillehei Heart Institute (LHI), University of Minnesota, Minneapolis, Minnesota, USA.
Daniel J GarryLillehei Heart Institute (LHI), University of Minnesota, Minneapolis, Minnesota, USA.
University of Minnesota · US

Funding

Mechanisms that Govern and Promote Cardiovascular RegenerationR01HL148599 · NHLBI · UNIVERSITY OF MINNESOTA · PI GARRY, DANIEL J., GARRY, MARY G · 2019 to 2022
$3.0M
NHLBI NIH HHS R01 HL148599
6 · The paper itself

Abstract

The ability to maintain viable cultures of mature, primary cardiomyocytes is challenging. The lack of viable cardiomyocyte cultures severely limits in vitro biochemical assays, toxicology assays, drug screening assays, and other analyses. Here, we describe a novel three-dimensional (3D) embryonic scaffold, which supports the culture of postnatal day 7 murine cardiomyocytes within the embryonic heart for, at least, 28 days. We have observed that these cardiomyocytes display normal differentiation, protein expression, and function after extended culture. This novel culture system will allow for prolonged treatment of cardiomyocytes in a natural 3D orientation and has the potential for providing a superior tool for the screening of therapeutic compounds.

Indexed as

Myocytes, CardiacTissue ScaffoldsAnimalsCell DifferentiationMiceembryonic scaffoldlong-term culturemature cardiomyocytes

Identifiers

PMID33843275
PMCPMC8309393
OpenAlexW3155666689

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.