ArticleACS synthetic biology2021
Optimized iLID Membrane Anchors for Local Optogenetic Protein Recruitment.
Article in ACS synthetic biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Reconstitution of Ras-PI3Kγ membrane communication and feedback using light-induced signaling inputs.Nature communications · 2026Article
- Interplay between Rac1/RhoA and actin waves in giant epithelial cells: Experiment and theory.Biophysical journal · 2026Article
- Local RhoA activation induces anillin-independent septin recruitment in interphase cells.Molecular biology of the cell · 2026Article
- Article
- Light-Controlled Membrane Fusion in Synthetic Cells.Life (Basel, Switzerland) · 2026Review
- Optogenetic engineering of synthetic and natural receptors: design principles, functional mechanisms and biomedical applications.Regenerative biomaterials · 2026Review
- Orthogonal RNA-regulated destabilization domains for three-color RNA imaging with minimal RNA perturbation.Nature methods · 2026Article
- Local RhoA activation induces septin recruitment.bioRxiv : the preprint server for biology · 2025Article
- Optogenetic actin network assembly on lipid bilayer uncovers the network density-dependent functions of actin-binding proteins.Nature communications · 2025Article
- Endogenous OptoRhoGEFs reveal biophysical principles of epithelial tissue furrowing.Nature communications · 2025Article
- A high-throughput platform for single-molecule tracking identifies drug interaction and cellular mechanisms.eLife · 2025Article
- Analysis of Light-Controlled Artificial Cell-Cell Adhesions.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Light-Based Juxtacrine Signaling Between Synthetic Cells.Small science · 2025Article
- Local monomer levels and established filaments potentiate non-muscle myosin 2 assembly.The Journal of cell biology · 2024Article
- Spatiotemporal Optical Control of Gαq-PLCβ Interactions.ACS synthetic biology · 2024Article
- Optogenetics: Illuminating the Future of Hearing Restoration and Understanding Auditory Perception.Current gene therapy · 2024Review
- Shining a light on RhoA: Optical control of cell contractility.The international journal of biochemistry & cell biology · 2023Review
- Article
- Computational framework for single-cell spatiotemporal dynamics of optogenetic membrane recruitment.Cell reports methods · 2022Article
- Design and engineering of light-sensitive protein switches.Current opinion in structural biology · 2022Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Optogenetic protein dimerization systems are powerful tools to investigate the biochemical networks that cells use to make decisions and coordinate their activities. These tools, including the improved Light-Inducible Dimer (iLID) system, offer the ability to selectively recruit components to subcellular locations, such as micron-scale regions of the plasma membrane. In this way, the role of individual proteins within signaling networks can be examined with high spatiotemporal resolution. Currently, consistent recruitment is limited by heterogeneous optogenetic component expression, and spatial precision is diminished by protein diffusion, especially over long time scales. Here, we address these challenges within the iLID system with alternative membrane anchoring domains and fusion configurations. Using live cell imaging and mathematical modeling, we demonstrate that the anchoring strategy affects both component expression and diffusion, which in turn impact recruitment strength, kinetics, and spatial dynamics. Compared to the commonly used C-terminal iLID fusion, fusion proteins with large N-terminal anchors show stronger local recruitment, slower diffusion of recruited components, efficient recruitment over wider gene expression ranges, and improved spatial control over signaling outputs. We also define guidelines for component expression regimes for optimal recruitment for both cell-wide and subcellular recruitment strategies. Our findings highlight key sources of imprecision within light-inducible dimer systems and provide tools that allow greater control of subcellular protein localization across diverse cell biological applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.