Evidence map›Paper›PMID 33843000›Full record

ArticleEnvironmental science and pollution research international2021

Enzyme polymer engineered structure strategy to enhance cross-linked enzyme aggregate stability: a step forward in laccase exploitation for cannabidiol removal from wastewater.

Arielle Farida Ariste, Lounes Haroune, Sabrina Saibi, Hubert Cabana

Abstract read
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In one paragraph

Article in Environmental science and pollution research international, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 13 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Arielle Farida AristeUniversité de Sherbrooke Water Research Group, Department of Civil and Building Engineering, Université de Sherbrooke, 2500 boul. de l'Université, Sherbrooke, Québec, J1K 2R1, Canada.
Lounes HarouneSherbrooke Pharmacology Institute, Université de Sherbrooke, Campus de la santé, 3001 12 Ave N, Sherbrooke, J1H 5N4, Québec, Canada.
Sabrina SaibiSherbrooke Pharmacology Institute, Université de Sherbrooke, Campus de la santé, 3001 12 Ave N, Sherbrooke, J1H 5N4, Québec, Canada.
Hubert CabanaUniversité de Sherbrooke Water Research Group, Department of Civil and Building Engineering, Université de Sherbrooke, 2500 boul. de l'Université, Sherbrooke, Québec, J1K 2R1, Canada. Hubert.Cabana@Usherbrooke.ca.ORCID http://orcid.org/0000-0003-3417-6816
Université de Sherbrooke · CA

Funding

Fonds Québécois de la Recherche sur la Nature et les Technologies 182383
6 · The paper itself

Abstract

Despite all its advantages and potential, cross-linking enzyme aggregate (CLEA) technology is still not applied at an industrial scale for enzyme insolubilization for bioremediation purposes. In this study, the enzyme polymer engineered structure (EPES) method was used to enhance CLEA stability and reuse. A crude laccase from Trametes hirsuta was successfully insolubilized to form EPES-CLEAs. The polymeric network provided excellent stability (> 90%) to CLEAs after a 24-h incubation in a non-buffered municipal wastewater effluent (WW), and the biocatalysts were recycled using a centrifugation process. While CLEAs activity dropped to 17%, EPES-CLEAs showed a laccase activity retention of 67% after five cycles of 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) oxidation. After 8 h of treatment in WW, the EPES-CLEAs were equally as effective in removing cannabidiol (CBD) as the free-LAC (~ 37%). This research demonstrates that the EPES method is a promising alternative for CLEA stabilization and reuse in environmental conditions.

Indexed as

CannabidiolLaccaseEnzymes, ImmobilizedEnzyme StabilityPolymersPolyporaceaeTrametesWastewaterCannabidiolEnzymes, ImmobilizedLaccasePolymersWastewaterBiocatalysisCannabinoidsLaccase insolubilizationPharmaceutical active compoundsWastewater treatmentWhite-rot-fungi

Identifiers

PMID33843000
OpenAlexW3157003043

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.