ArticleWater research X2021
Co-quantification of crAssphage increases confidence in wastewater-based epidemiology for SARS-CoV-2 in low prevalence areas.
Article in Water research X, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers, 4 of them syntheses that pooled it.
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The trial behind it
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Who cites it
68 citing papers in PubMed, 4 syntheses or guidelines pooled it, 136 citations in OpenAlex.
- Current state and future perspectives on de facto population markers for normalization in wastewater-based epidemiology: A systematic literature review.The Science of the total environment · 2024Pooled it
- SARS-CoV-2 shedding sources in wastewater and implications for wastewater-based epidemiology.Journal of hazardous materials · 2022Pooled it
- Factors influencing SARS-CoV-2 RNA concentrations in wastewater up to the sampling stage: A systematic review.The Science of the total environment · 2022Pooled it
- Wastewater surveillance to infer COVID-19 transmission: A systematic review.The Science of the total environment · 2022Pooled it
- Correlation of Norovirus in Wastewater with Gastroenteritis-Related Hospitalizations, New York, USA, 2022-2024.Emerging infectious diseases · 2026Article
- Estimating wastewater concentrations of norovirus and rotavirus from global data on community-level infection prevalence and viral shedding.Journal of water and health · 2026Article
- Incidence and temporal distribution of SARS-CoV-2 circulating in the municipal wastewaters of the Buffalo City region, Eastern Cape, South Africa.Global epidemiology · 2025Article
- Recovering wastewater RNA for virome sequencing by systematically optimized tangential-flow ultrafiltration and Nanotrap microbiome particles.Applied and environmental microbiology · 2025Article
- Wastewater Surveillance for Group APathogens (Basel, Switzerland) · 2025Article
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- SARS-CoV-2 viral titer measurements in Ontario, Canada wastewaters throughout the COVID-19 pandemic.Scientific data · 2024Article
- CrAss-Like Phages: From Discovery in Human Fecal Metagenome to Application as a Microbial Source Tracking Marker.Food and environmental virology · 2024Review
- Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond.Clinical microbiology reviews · 2024Review
- Simple SARS-CoV-2 concentration methods for wastewater surveillance in low resource settings.The Science of the total environment · 2024Article
- Clinically relevant sequence types of carbapenemase-producing Escherichia coli and Klebsiella pneumoniae detected in Finnish wastewater in 2021-2022.Antimicrobial resistance and infection control · 2024Article
- Precision public health in schools enabled by wastewater surveillance: A case study of COVID-19 in an Upstate New York middle-high school campus during the 2021-2022 academic year.PLOS global public health · 2024Article
- Non-detection of emerging and re-emerging pathogens in wastewater surveillance to confirm absence of transmission risk: A case study of polio in New York.PLOS global public health · 2024Article
- Genomic profiling and spatial SEIR modeling of COVID-19 transmission in Western New York.Frontiers in microbiology · 2024Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wastewater surveillance of SARS-CoV-2 RNA is increasingly being incorporated into public health efforts to respond to the COVID-19 pandemic. In order to obtain the maximum benefit from these efforts, approaches to wastewater monitoring need to be rapid, sensitive, and relatable to relevant epidemiological parameters. In this study, we present an ultracentrifugation-based method for the concentration of SARS-CoV-2 wastewater RNA and use crAssphage, a bacteriophage specific to the human gut, to help account for RNA loss during transit in the wastewater system and sample processing. With these methods, we were able to detect, and sometimes quantify, SARS-CoV-2 RNA from 20 mL wastewater samples within as little as 4.5 hours. Using known concentrations of bovine coronavirus RNA and deactivated SARS-CoV-2, we estimate recovery rates of approximately 7-12% of viral RNA using our method. Results from 24 sewersheds across Upstate New York during the spring and summer of 2020 suggested that stronger signals of SARS-CoV-2 RNA from wastewater may be indicative of greater COVID-19 incidence in the represented service area approximately one week in advance. SARS-CoV-2 wastewater RNA was quantifiable in some service areas with daily positives tests of less than 1 per 10,000 people or when weekly positive test rates within a sewershed were as low as 1.7%. crAssphage DNA concentrations were significantly lower during periods of high flow in almost all areas studied. After accounting for flow rate and population served, crAssphage levels per capita were estimated to be about 1.35 × 10
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.