ArticleFrontiers in cellular and infection microbiology2021
Direct Detection and Identification of the Most Common Bacteria and Fungi Causing Otitis Externa by a Stepwise Multiplex PCR.
Article in Frontiers in cellular and infection microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 3 of them syntheses that pooled it.
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Who cites it
32 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Nucleic Acid Amplification Tests forPathogens (Basel, Switzerland) · 2026Pooled it
- Otomycosis: a systematic review and meta-analysis of prevalence and causative agents in the era of molecular diagnostics.BMC infectious diseases · 2025Pooled it
- Prevalence of bacterial ear infections and multidrug resistance patterns among ear infection suspected patients in Ethiopia: a systematic review and meta-analysis.BMC infectious diseases · 2024Pooled it
- Optimizing pathogen detection in skull base osteomyelitis: a comparative analysis of swab versus biopsy sampling.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2026Article
- Development of a recombinase polymerase amplification-based chromogenic assay for rapid detection ofFrontiers in microbiology · 2026Article
- Lineage-specific endosymbiosis in Mucorales: restriction ofCurrent research in microbial sciences · 2026Article
- Preadmission systemic antibiotic treatment in necrotizing otitis externa (NOE).European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2025Article
- Molecular Assays Versus Mycological Methods for Diagnosis of Rhino Orbital Mucormycosis: Analysis of 120 Clinical Specimens from COVID-19 Patients.Mycopathologia · 2025Article
- Cross-kingdom pathogen detection via duplex universal PCR and high-resolution melt.Biosensors & bioelectronics · 2025Article
- Culture-Dependent and -Independent Wastewater Surveillance for Multiple Pathogenic Yeasts.Journal of fungi (Basel, Switzerland) · 2025Article
- Current Updates on Molecular Diagnostic Assays Used for Detection ofDiagnostics (Basel, Switzerland) · 2025Review
- The Use of Gel Electrophoresis to Separate Multiplex Polymerase Chain Reaction Amplicons Allows for the Easy Identification and Assessment of the Spread of ToxigenicGels (Basel, Switzerland) · 2024Article
- Aflatoxin profiles of Aspergillus flavus isolates in Sudanese fungal rhinosinusitis.Medical mycology · 2024Article
- Yeast species in the respiratory samples of COVID-19 patients; molecular tracking ofFrontiers in cellular and infection microbiology · 2024Article
- The role of the microbiome in allergic dermatitis-related otitis externa: a multi-species comparative review.Frontiers in veterinary science · 2024Review
- Fungi involved in rhinosinusitis in arid regions: insights from molecular identification and antifungal susceptibility.Microbiology spectrum · 2023Article
- A case of COVID-19-associated mucormycosis due to Lichtheimia ramosa.Journal of clinical laboratory analysis · 2023Article
- An Overview of the Public Health Challenges in Diagnosing and Controlling Human Foodborne Pathogens.Vaccines · 2023Review
- A Comprehensive Review of Identification Methods for Pathogenic Yeasts: Challenges and Approaches.Advanced biomedical research · 2023Review
- Case report: COVID-19-associated mucormycosis co-infection withFrontiers in medicine · 2023Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Considering the importance of differential diagnosis of infectious otitis externa (OE), a stepwise PCR-based assay using universal and genus- or species-specific primers for the detection/identification of the most prevalent bacterial and fungal OE was developed and evaluated on the ear aspiration specimens of clinically suspected patients. Methods and Materials: A total of 120 ear aspiration specimens with otomycosis suspicion were subjected to manual DNA extraction using phenol-chloroform extraction after tissue digestion with a lysis buffer. The multiplex PCR was initially performed using pan-fungal and bacterial homemade primers. Results: The overall multiplex PCR-based detection rate of bacterial (n = 88; 73.3%) and fungal (n = 97; 81%) OE was documented to be 100% along with and complete consistency with the results of direct examination and Giemsa staining. Double amplicon bands of bacterial and fungal pathogens were evidenced in 76 specimens (63.3%). Moreover, the positivity rate of pan-fungal PCR was higher than that of the culture result. Out of 88 pan-bacterial positive PCR specimens, 66 and 47 ones were positive for Conclusion: The stepwise multiplex PCR assay proved to be more sensitive, more rapid, as well as less cumbersome in detection and identification of fungal and bacterial OE, compared to culture.
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