Plug-in tubes allow tunable oil removal, droplet packing, and reaction incubation for time-controlled droplet-based assays.

Meng Sun, Gembu Maryu, Shiyuan Wang, Qiong Yang, Ryan C Bailey

Abstract read

In one paragraph

Article in Biomicrofluidics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Meng SunORCID 0000-0002-0097-8012

Gembu MaryuDepartment of Biophysics, University of Michigan, Ann Arbor, Michigan 48109, USA.ORCID 0000-0003-2023-8377

Shiyuan WangDepartment of Biophysics, University of Michigan, Ann Arbor, Michigan 48109, USA.ORCID 0000-0001-8676-8720

Qiong YangDepartment of Biophysics, University of Michigan, Ann Arbor, Michigan 48109, USA.ORCID 0000-0002-2442-2094

Ryan C BaileyDepartment of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA.ORCID 0000-0003-1021-4267

Funding

Supplement: Quantitative in vitro and in vivo analyses of oscillatory processes in early zebrafish embryosR35GM119688 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI YANG, QIONG · 2016 to 2020

$1.9M

Droplet Microfluidic Platform for Ultralow Input EpigeneticsR21CA191186 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BAILEY, RYAN C · 2015 to 2017

$647k

NCI NIH HHS R21 CA191186NIGMS NIH HHS R35 GM119688

6 · The paper itself

Abstract

Here, we report a unique microfluidic technique that utilizes a membrane filter and plug-in tubes to remove oil and pack water-in-oil droplets for controlled incubation of droplet-based assays. This technique could be modularly incorporated into most droplet-generation devices without a need to alter the original designs. Our results show that removing excess oil to form tightly packed droplets allows for extended and controllable incubation for droplets traveling in microchannels. The efficiency of this technique was evaluated and confirmed using a time-dependent enzyme assay with a fluorometric readout. The system is also readily generalizable to control inter-droplet distance, crucial for studying droplet communication and pattern formation.

Identifiers

PMID33841602

DOI10.1063/5.0047924

PMCPMC8024030

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.