ArticleSensors and actuators. B, Chemical2021
Rapid point-of-care testing for SARS-CoV-2 virus nucleic acid detection by an isothermal and nonenzymatic Signal amplification system coupled with a lateral flow immunoassay strip.
Article in Sensors and actuators. B, Chemical, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Accuracy of novel antigen rapid diagnostics for SARS-CoV-2: A living systematic review and meta-analysis.PLoS medicine · 2021Pooled it
- An Ultrasensitive Fluorescent Immunoassay Based on R-CDs@SiOApplied biochemistry and biotechnology · 2026Article
- Rapid and facile detection of Mycoplasma pneumoniae 16S rRNA based on CHA-FICA system.Mikrochimica acta · 2025Article
- Lateral flow assays: Progress and evolution of recent trends in point-of-care applications.Materials today. Bio · 2024Review
- Gold Nanoparticle-Based Colorimetric and Fluorescent Dual-Mode Lateral Flow Immunoassay for SARS-CoV-2 Detection.Journal of functional biomaterials · 2024Article
- Quantitative mRNA expression measurement at home.Scientific reports · 2024Article
- A colorimetric lateral flow immunoassay based on oriented antibody immobilization for sensitive detection of SARS-CoV-2.Sensors and actuators. B, Chemical · 2023Article
- Nanotechnology-Assisted Biosensors for the Detection of Viral Nucleic Acids: An Overview.Biosensors · 2023Review
- Recent progress in the development of DNA-based biosensors integrated with hybridization chain reaction or catalytic hairpin assembly.Frontiers in chemistry · 2023Review
- An ultra-sensitive and specific nanoplasmonic-enhanced isothermal amplification platform for the ultrafast point-of-care testing of SARS-CoV-2.Chemical engineering journal (Lausanne, Switzerland : 1996) · 2023Article
- Hijacking the self-replicating machine of bacteriophage for PCR-based cascade signal amplification in detecting SARS-CoV-2 viral marker protein in serum.Sensors and actuators. B, Chemical · 2023Article
- Detection of Hepatitis C virus RNA using a novel hybridization chain reaction method that competitively dampens cascade amplification.PloS one · 2023Article
- Evaluation of Four Strategies for SARS-CoV-2 Detection: Characteristics and Prospects.Microbiology spectrum · 2022Article
- Nanozyme-strip for rapid and ultrasensitive nucleic acid detection of SARS-CoV-2.Biosensors & bioelectronics · 2022Article
- Lateral flow assays for viruses diagnosis: Up-to-date technology and future prospects.Trends in analytical chemistry : TRAC · 2022Review
- Article
- Detection ofACS omega · 2022Article
- Diagnosis Methods for COVID-19: A Systematic Review.Micromachines · 2022Review
- Toward smart diagnosis of pandemic infectious diseases using wastewater-based epidemiology.Trends in analytical chemistry : TRAC · 2022Review
- Advancements in Testing Strategies for COVID-19.Biosensors · 2022Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An outbreak of a new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), began in December 2019. Accurate, rapid, convenient, and relatively inexpensive diagnostic methods for SARS-CoV-2 infection are important for public health and optimal clinical care. The current gold standard for diagnosing SARS-CoV-2 infection is reverse transcription-polymerase chain reaction (RT-PCR). However, RTPCR assays are designed for use in well-equipped laboratories with sophisticated laboratory infrastructure and highly trained technicians, and are unsuitable for use in under-equipped laboratories and in the field. In this study, we report the development of an accurate, rapid, and easy-to-implement isothermal and nonenzymatic signal amplification system (a catalytic hairpin assembly (CHA) reaction) coupled with a lateral flow immunoassay (LFIA) strip-based detection method that can detect SARSCoV-2 in oropharyngeal swab samples. Our method avoids RNA isolation, PCR amplification, and elaborate result analysis, which typically takes 6-8 h. The entire CHA-LFIA detection method, from nasopharyngeal sampling to obtaining test results, takes less than 90 min. Such methods are simple and require no expensive equipment, only a simple thermostatically controlled water bath and a fluorescence reader device. We validated our method using synthetic oligonucleotides and clinical samples from 15 patients with SARS-CoV-2 infection and 15 healthy individuals. Our detection method provides a fast, simple, and sensitive (with a limit of detection (LoD) of 2000 copies/mL) alternative to the SARS-CoV-2 RT-PCR assay, with 100 % positive and negative predictive agreements.
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