Evidence map›Paper›PMID 33840009›Full record

ArticleThe protein journal2021

Label-Free Method Development for Hydroxyproline PTM Mapping in Human Plasma Proteome.

Debabrata Dutta, Shakilur Rahman, Gourab Bhattacharje, Swarnendu Bag, Bidhan Chandra Sing, Jyotirmoy Chatterjee, Amit Basak, Amit Kumar Das

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Article in The protein journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Debabrata DuttaDepartment of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
Shakilur Rahman *Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
Gourab Bhattacharje *Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
Swarnendu BagSchool of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
Bidhan Chandra SingCentral Research Facility, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
Jyotirmoy ChatterjeeSchool of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
Amit BasakDepartment of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
Amit Kumar DasDepartment of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India. amitk@bt.iitkgp.ac.in.ORCID 0000-0001-9703-1898
Indian Institute of Technology Kharagpur · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Post-translational modifications (PTMs) impart structural heterogeneities that can alter plasma proteins' functions in various pathophysiological processes. However, the identification and mapping of PTMs in untargeted plasma proteomics is still a challenge due to the presence of diverse components in blood. Here, we report a label-free method for identifying and mapping hydroxylated proteins using tandem mass spectrometry (MS/MS) in the human plasma sample. Our untargeted proteomics approach led us to identify 676 de novo sequenced peptides in human plasma that correspond to 201 proteins, out of which 11 plasma proteins were found to be hydroxylated. Among these hydroxylated proteins, Immunoglobulin A1 (IgA1) heavy chain was found to be modified at residue 285 (Pro

Indexed as

Blood ProteinsHydroxyprolineProtein Processing, Post-TranslationalProteomeProteomicsChromatography, LiquidHumansTandem Mass SpectrometryBlood ProteinsHydroxyprolineProteomeHydroxyprolineImmunoglobulinLabel-free methodsMolecular dynamics simulationMS/MSPlasma proteomicsPost-translational modifications

Identifiers

PMID33840009
OpenAlexW3156335044

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.