ArticleBMC plant biology2021
Cold acclimation can specifically inhibit chlorophyll biosynthesis in young leaves of Pakchoi.
Article in BMC plant biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Convergent roles of BcGUN4.1, BcSG1, BcCHLH, and BcTPR4 in regulating the leaf greenness of non-heading Chinese cabbage.Molecular horticulture · 2026Article
- ZnONPs Alleviates Salt Stress in Maize Seedlings by Improving Antioxidant Defense and Photosynthesis Potential.Plants (Basel, Switzerland) · 2025Article
- The chloroplast 16S rRNA dimethyltransferase BrPFC1 is required for Brassica rapa development under chilling stress.The Plant journal : for cell and molecular biology · 2025Article
- The Scarlet Alchemy of Survival: Integrated Transcriptomic and Metabolomic Analysis of Leaf Coloration in EndangeredPlants (Basel, Switzerland) · 2025Article
- Review
- Biosynthesis and Extraction of Chlorophyll, Carotenoids, Anthocyanins, and Betalaine In Vivo and In Vitro.Current issues in molecular biology · 2024Review
- Cold acclimation alleviates photosynthetic inhibition and oxidative damage induced by cold stress in citrus seedlings.Plant signaling & behavior · 2023Article
- Prolong the postharvest shelf life of spinach through the antioxidative ability of melatonin.Food chemistry: X · 2023Article
- Function of ALA Content in Porphyrin Metabolism Regulation ofInternational journal of molecular sciences · 2023Article
- Understanding and Comprehensive Evaluation of Cold Resistance in the Seedlings of Multiple Maize Genotypes.Plants (Basel, Switzerland) · 2022Article
- Recent applications of metabolomics in plant breeding.Breeding science · 2022Article
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Authors and funding
6 authors at 4 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundLeaf color is an important trait in breeding of leafy vegetables. Y-05, a pakchoi (Brassica rapa ssp. chinensis) cultivar, displays yellow inner (YIN) and green outer leaves (GOU) after cold acclimation. However, the mechanism of this special phenotype remains elusive.
resultsWe assumed that the yellow leaf phenotype of Y-05 maybe caused by low chlorophyll content. Pigments measurements and transmission electron microscopy (TEM) analysis showed that the yellow phenotype is closely related with decreased chlorophyll content and undeveloped thylakoids in chloroplast. Transcriptomes and metabolomes sequencing were next performed on YIN and GOU. The transcriptomes data showed that 4887 differentially expressed genes (DEGs) between the YIN and GOU leaves were mostly enriched in the chloroplast- and chlorophyll-related categories, indicating that the chlorophyll biosynthesis is mainly affected during cold acclimation. Together with metabolomes data, the inhibition of chlorophyll biosynthesis is contributed by blocked 5-aminolevulinic acid (ALA) synthesis in yellow inner leaves, which is further verified by complementary and inhibitory experiments of ALA. Furthermore, we found that the blocked ALA is closely associated with increased BrFLU expression, which is indirectly altered by cold acclimation. In BrFLU-silenced pakchoi Y-05, cold-acclimated leaves still showed green phenotype and higher chlorophyll content compared with control, meaning silencing of BrFLU can rescue the leaf yellowing induced by cold acclimation.
conclusionsOur findings suggested that cold acclimation can indirectly promote the expression of BrFLU in inner leaves of Y-05 to block ALA synthesis, resulting in decreased chlorophyll content and leaf yellowing. This study revealed the underlying mechanisms of leaves color change in cold-acclimated Y-05.
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