Evidence map›Paper›PMID 33837745›Full record

ArticleThe Journal of biological chemistry

Conserved binding site in the N-lobe of prokaryotic MATE transporters suggests a role for Na

Steven Castellano, Derek P Claxton, Emel Ficici, Tsukasa Kusakizako, Robyn Stix, Wenchang Zhou, Osamu Nureki, Hassane S Mchaourab, José D Faraldo-Gómez

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Steven CastellanoTheoretical Molecular Biophysics Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA.
Derek P ClaxtonDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA. Electronic address: derek.p.claxton@vanderbilt.edu.
Emel FiciciTheoretical Molecular Biophysics Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA.
Tsukasa KusakizakoDepartment of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Robyn StixTheoretical Molecular Biophysics Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA.
Wenchang ZhouTheoretical Molecular Biophysics Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA.
Osamu NurekiDepartment of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Hassane S MchaourabDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
José D Faraldo-GómezTheoretical Molecular Biophysics Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA. Electronic address: jose.faraldo@nih.gov.

Funding

Molecular Mechanisms in Membrane Bioenergetics, Transport and SignalingZIAHL006203 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI FARALDO-GÓMEZ, JOSE · 2015 to 2023
$15.4M
Medical Scientist Training Program Training GrantT32GM145449 · NIGMS · DUKE UNIVERSITY · PI Christopher D Kontos · 2022 to 2026
$6.6M
Structural Dynamics of Multi-drug TransportersR01GM077659 · NIGMS · VANDERBILT UNIVERSITY · PI MCHAOURAB, HASSANE S · 2006 to 2023
$5.0M
NIGMS NIH HHS R01 GM077659NIGMS NIH HHS T32 GM145449
6 · The paper itself

Abstract

In both prokaryotes and eukaryotes, multidrug and toxic-compound extrusion (MATE) transporters catalyze the efflux of a broad range of cytotoxic compounds, including human-made antibiotics and anticancer drugs. MATEs are secondary-active antiporters, i.e., their drug-efflux activity is coupled to, and powered by, the uptake of ions down a preexisting transmembrane electrochemical gradient. Key aspects of this mechanism, however, remain to be delineated, such as its ion specificity and stoichiometry. We previously revealed the existence of a Na

Indexed as

Anti-Bacterial AgentsAntineoplastic AgentsAntiportersBinding SitesCrystallography, X-RayHumansIonsModels, MolecularMolecular Dynamics SimulationOrganic Cation Transport ProteinsProkaryotic CellsProtein ConformationProtein DomainsSodiumAnti-Bacterial AgentsAntineoplastic AgentsAntiportersIonsOrganic Cation Transport ProteinsSodiumdouble electron–electron resonance spectroscopydrug-efflux pumpsion selectivitymolecular dynamicsmultidrug resistancesecondary-active transporters

Identifiers

PMID33837745
PMCPMC7949106

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.