Evidence map›Paper›PMID 33837517›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Human Induced Pluripotent Stem Cell (iPSC) Handling Protocols: Maintenance, Expansion, and Cryopreservation.

Davide Marotta, Chandrika Rao, Valentina Fossati

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
4.2field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. Review
  3. GMP Manufacturing of Allogenic iPSC-NK Cells for Immunotherapy.Advances in experimental medicine and biology · 2025
    Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Davide MarottaThe New York Stem Cell Foundation Research Institute, New York, NY, USA.
Chandrika RaoThe New York Stem Cell Foundation Research Institute, New York, NY, USA.
Valentina FossatiThe New York Stem Cell Foundation Research Institute, New York, NY, USA. vfossati@nyscf.org.
New York Stem Cell Foundation · US

Funding

Use of iPSC systems to define roles of microglial TREM2/DAP12 and CR3/DAP12 complexes and their genetic variants in specifying risk for late onset sporadic Alzheimer's diseaseR01AG061894 · NIA · NEW YORK STEM CELL FOUNDATION · PI FOSSATI, VALENTINA, GANDY, SAMUEL E. · 2018 to 2022
$4.2M
Investigating the biology of human neurotoxic reactive astrocytes using induced pluripotent stem cellsR21NS111186 · NINDS · NEW YORK STEM CELL FOUNDATION · PI FOSSATI, VALENTINA · 2019 to 2019
$521k
NIA NIH HHS R01 AG061894NINDS NIH HHS R21 NS111186
6 · The paper itself

Abstract

Human induced pluripotent stem cells (iPSCs) have emerged as an invaluable resource for basic research, disease modeling, and drug discovery over recent years. Given the numerous advantages of iPSCs over alternative models-including their human origin, their ability to be differentiated into almost any cell type, and the therapeutic potential of patient-specific iPSCs in personalized medicine-many labs are now considering iPSC models for their studies. As the quality of the starting population of iPSCs is a key determinant in the success of any one of these applications, it is crucial to adhere to best practices in iPSC culture. In the following protocol, we offer a comprehensive guide to the culture, cryopreservation, and quality control methods required for the establishment and maintenance of high-quality iPSC cultures.

Indexed as

Induced Pluripotent Stem CellsCell DifferentiationCryopreservationHumansPrecision MedicineCell cultureCryopreservationHuman induced pluripotent stem cellsPluripotency

Identifiers

PMID33837517
OpenAlexW3150874388

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.