ArticleApplied and environmental microbiology2021
Biosensor-Based Multigene Pathway Optimization for Enhancing the Production of Glycolate.
Article in Applied and environmental microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- Balancing Cell Growth and Product Synthesis for Efficient Microbial Cell Factories.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Computation-aided designs enable developing auxotrophic metabolic sensors for wide-range glyoxylate and glycolate detection.Nature communications · 2025Article
- Fermentation design and process optimization strategy based on machine learning.Biodesign research · 2025Review
- Genetically encoded biosensors for the circular plastics bioeconomy.Metabolic engineering communications · 2024Article
- Biotransformation of ethylene glycol by engineeredSynthetic and systems biotechnology · 2024Article
- Article
- Genetically Encoded Biosensor Engineering for Application in Directed Evolution.Journal of microbiology and biotechnology · 2023Review
- Tunable T7 Promoter Orthogonality on T7RNAP forACS synthetic biology · 2023Article
- Biosensor-based high-throughput screening enabled efficient adipic acid production.Applied microbiology and biotechnology · 2023Article
- Improving the production of the micafungin precursor FR901379 in an industrial production strain.Microbial cell factories · 2023Article
- Chemoenzymatic conversion of glycerol to lactic acid and glycolic acid.Bioresources and bioprocessing · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycolate is widely used in industry, especially in the fields of chemical cleaning, cosmetics, and medical materials, and has broad market prospects for the future. Recent advances in metabolic engineering and synthetic biology have significantly improved the titer and yield of glycolate. However, an expensive inducer was used in previous studies, which is not feasible for use in large-scale industrial fermentations. To constitutively biosynthesize glycolate, the expression level of each gene of the glycolate synthetic pathway needs to be systemically optimized. The main challenge of multigene pathway optimization is being able to select or screen the optimum strain from the randomly assembled library by an efficient high-throughput method within a short time. To overcome these challenges, we firstly established a glycolate-responsive biosensor and developed agar plate- and 48-well deep-well plate-scale high-throughput screening methods for the rapid screening of superior glycolate producers from a large library. A total of 22 gradient-strength promoter-5'-untranslated region (UTR) complexes were randomly cloned upstream of the genes of the glycolate synthetic pathway, generating a large random assembled library. After rounds of screening, the optimum strain was obtained from 6 × 10
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