Evidence map›Paper›PMID 33835421›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2021

Complete genome sequence and analysis of a Saccharomyces cerevisiae strain used for sugarcane spirit production.

Ane Catarine Tosi Costa, Jacob Hornick, Tathiana Ferreira Sá Antunes, Alexandre Martins Costa Santos, A Alberto R Fernandes, James R Broach, Patricia M B Fernandes

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Ane Catarine Tosi CostaPrograma de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitoria, ES, 29040-090, Brazil.ORCID https://orcid.org/0000-0002-2521-9653
Jacob HornickDepartment of Biochemistry and Molecular Biology, Penn State University College of Medicine, 500 University Drive, Hershey, PA, 17033, USA.ORCID https://orcid.org/0000-0002-0862-4235
Tathiana Ferreira Sá AntunesPrograma de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitoria, ES, 29040-090, Brazil.ORCID https://orcid.org/0000-0001-5551-5958
Alexandre Martins Costa SantosPrograma de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitoria, ES, 29040-090, Brazil.ORCID https://orcid.org/0000-0002-8801-8875
A Alberto R FernandesPrograma de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitoria, ES, 29040-090, Brazil.ORCID https://orcid.org/0000-0002-8261-9717
James R BroachDepartment of Biochemistry and Molecular Biology, Penn State University College of Medicine, 500 University Drive, Hershey, PA, 17033, USA.ORCID https://orcid.org/0000-0003-1197-0312
Patricia M B FernandesPrograma de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitoria, ES, 29040-090, Brazil. patricia.fernandes@ufes.br.ORCID http://orcid.org/0000-0003-2695-3638
Universidade Federal do Espírito Santo · BRPennsylvania State University · US

Funding

Ras and TOR Signaling in YeastR01GM076562 · NIGMS · PRINCETON UNIVERSITY · PI BROACH, JAMES R. · 2007 to 2019
$5.0M
Conselho Nacional de Desenvolvimento Científico e Tecnológico 458029/2014-9NIGMS NIH HHS R01 GM076562NIH HHS GM076562
6 · The paper itself

Abstract

Distillation of fermented sugarcane juice produces both rum and cachaça, significant sources of revenue in Brazil and elsewhere. In this study, we provide a genomic analysis of a Saccharomyces cerevisiae strain isolated from a cachaça distillery in Brazil. We determined the complete genome sequence of a strain with high flocculation capacity, high tolerance to ethanol, osmotic and heat shock stress and high fermentation rates and compared the sequence with that of the reference S288c genome as well as those of two other cachaça strains. Single-nucleotide polymorphism analysis identified alterations in genes involved in nitrogen and organic compound metabolism, integrity of organelles and ion homeostasis. The strain exhibited fragmentation of several flocculation genes relative to the reference genome, as well as loss of a stop codon in the FLO8 gene, which encodes a transcription factor required for FLO gene expression. The strain contained no genes not present in the reference genome strain but did lack several genes, including asparaginase genes, maltose utilization loci, and several genes from the tandem array of the DUP240 family. The three cachaça strains lacked different sets of genes, but the asparaginase genes and several of the DUP240 genes were common deficiencies. This study provides new insights regarding the selective pressure of sugarcane fermentation on the genome of yeast strains and offers additional genetic resources for modern synthetic biology and genome editing tools.

Indexed as

Genome, FungalSaccharomyces cerevisiaeSaccharumAsparaginaseEthanolFermentationFermented BeveragesAsparaginaseEthanolCachaça productionFermentative processWhole-genome sequencingYeast

Identifiers

PMID33835421
PMCPMC8324658
OpenAlexW3143634679

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.