ArticleFolia microbiologica2021
Effects of DNA preservation solution and DNA extraction methods on microbial community profiling of soil.
Article in Folia microbiologica, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 38 citations in OpenAlex.
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- Microbiomes of frozen blood plasma samples reveal potential pathogens in wild birds and rodents.One health (Amsterdam, Netherlands) · 2025Article
- Microbial community characterization in Red Sea-derived samples using a field-deployable DNA extraction system and nanopore sequencing.Environmental microbiome · 2025Article
- Desiccation as a suitable alternative to cold-storage of phyllosphere samples for DNA-based microbial community analyses.Scientific reports · 2025Article
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- Drying as an effective method to store soil samples for DNA-based microbial community analyses: a comparative study.Scientific reports · 2024Article
- Comparative evaluation of soil DNA extraction kits for long read metagenomic sequencing.Access microbiology · 2024Article
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- Correlating the succession of microbial communities from Nigerian soils to petroleum biodegradation.World journal of microbiology & biotechnology · 2023Article
- Exploring the Association between Citrus Nutraceutical Eriocitrin and Metformin for Improving Pre-Diabetes in a Dynamic Microbiome Model.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Exploring the Potential ofNutrients · 2023Article
- Oil Absorbent Polypropylene Particles Stimulate Biodegradation of Crude Oil by Microbial Consortia.Frontiers in microbiology · 2022Article
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Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
Microbial community profiling using high-throughput sequencing relies in part on the preservation of the DNA and the effectiveness of the DNA extraction method. This study aimed at understanding to what extent these parameters affect the profiling. We obtained samples treated with and without a preservation solution. Also, we compared DNA extraction kits from Qiagen and Zymo-Research. The types of samples were defined strains, both as single species and mixtures, as well as undefined indigenous microbial communities from soil. We show that the use of a preservation solution resulted in substantial changes in the 16S rRNA gene profiles either due to an overrepresentation of Gram-positive bacteria or to an underrepresentation of Gram-negative bacteria. In addition, 16S rRNA gene profiles were substantially different depending on the type of kit that was used for extraction. The kit from Zymo extracted DNA from different types of bacteria in roughly equal amounts. In contrast, the kit from Qiagen preferentially extracted DNA from Gram-negative bacteria while DNA from Gram-positive bacteria was extracted less effectively. These differences in kit performance strongly influenced the interpretation of our microbial ecology studies.
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Registered trials
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