Evidence map›Paper›PMID 33834358›Full record

ArticleMolecular biology reports2021

Understanding the regulatory relationship of abscisic acid and bZIP transcription factors towards amylose biosynthesis in wheat.

Pankaj Kumar, Afsana Parveen, Himanshu Sharma, Mohammed Saba Rahim, Ankita Mishra, Prashant Kumar, Koushik Shah, Vikas Rishi, Joy Roy

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Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.5field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Pankaj Kumar *National Agri-Food Biotechnology Institute, Knowledge City Sector-81, Mohali, Punjab, 140306, India.
Afsana Parveen *National Agri-Food Biotechnology Institute, Knowledge City Sector-81, Mohali, Punjab, 140306, India.
Himanshu SharmaNational Agri-Food Biotechnology Institute, Knowledge City Sector-81, Mohali, Punjab, 140306, India.
Mohammed Saba RahimNational Agri-Food Biotechnology Institute, Knowledge City Sector-81, Mohali, Punjab, 140306, India.
Ankita MishraNational Agri-Food Biotechnology Institute, Knowledge City Sector-81, Mohali, Punjab, 140306, India.
Prashant KumarNational Agri-Food Biotechnology Institute, Knowledge City Sector-81, Mohali, Punjab, 140306, India.
Koushik ShahNational Agri-Food Biotechnology Institute, Knowledge City Sector-81, Mohali, Punjab, 140306, India.
Vikas RishiNational Agri-Food Biotechnology Institute, Knowledge City Sector-81, Mohali, Punjab, 140306, India.
Joy RoyNational Agri-Food Biotechnology Institute, Knowledge City Sector-81, Mohali, Punjab, 140306, India. joykroy@nabi.res.in.ORCID http://orcid.org/0000-0001-5441-3538
National Agri-Food Biotechnology Institute · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Starch is biosynthesized during seed development and this process is regulated by many bZIP proteins in bread wheat. Abscisic acid (ABA), an important phyto-hormone involved in various physiological processes mediated by bZIPs in plants including seed development. The 'Group A' TabZIP transcription factors play important roles in the ABA signaling pathway in Arabidopsis, rice and other cereal crops but their role in regulation of amylose biosynthesis in wheat is limited. In this study 83 'Group A' TabZIPs were characterized by gene expression analysis in wheat amylose mutants. A set of 17 TabZIPs was selected on the basis of differential expression (> 2 fold) in low and high amylose mutants from RNA-seq data and validated by qRT PCR. Based on qRT PCR and correlation analysis out of the 17 TabZIPs six differentially expressed candidate TabZIPs were identified, involving in high amylose biosynthesis. The TabZIP175.2, identified as upregulated in all high amylose lines and TabZIP90.2, TabZIP129.1, TabZIP132.2, TabZIP143 and TabZIP159.2 were found downregulated in all low amylose lines, after exogenous supply of ABA. Proximal promoter analysis of starch pathway genes revealed the presence of ABA-responsive elements (ABREs) that are putative binding sites for bZIPs. Collectively, these findings indicated the involvement of putative six candidate TabZIPs as transcriptional regulators of amylose related genes via an ABA-dependent pathway in wheat. This study could help the investigators to make an informed decision to edit wheat genome for high/low amylose content using gene-editing technologies.

Indexed as

Abscisic AcidAmyloseBase SequenceBasic-Leucine Zipper Transcription FactorsBinding SitesBiosynthetic PathwaysGene Expression Regulation, PlantGenetic Association StudiesMutationResponse ElementsSignal TransductionStarchTranscriptomeTriticumAbscisic AcidAmyloseBasic-Leucine Zipper Transcription FactorsStarchABAAmylosebZIP-transcription factorTranscriptomeWheat

Identifiers

PMID33834358
OpenAlexW3148533396

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.