Evidence map›Paper›PMID 33834074›Full record

ArticleBioMed research international2021

Osteosarcoma Cell-Derived Exosomal miR-1307 Promotes Tumorgenesis via Targeting AGAP1.

Fei Han, Peidong Pu, Chao Wang, Xiao Ding, Zhoujun Zhu, Wei Xiang, Weishan Wang

Expression of concernOpen access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioMed research international, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
3.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 30 citations in OpenAlex.

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  13. Cellular and Genetic Background of Osteosarcoma.Current issues in molecular biology · 2023
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  16. Article
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  18. Role of extracellular vesicles in osteosarcoma.International journal of medical sciences · 2022
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Fei HanDepartment of Orthopaedics, The First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, China.
Peidong PuDepartment of Orthopaedics, The First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, China.
Chao WangDepartment of Orthopaedics, The First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, China.
Xiao DingDepartment of Orthopaedics, The First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, China.
Zhoujun ZhuDepartment of Orthopaedics, The First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, China.
Wei XiangDepartment of Orthopaedics, The First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, China.
Weishan WangDepartment of Orthopaedics, The First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, China.ORCID https://orcid.org/0000-0002-3945-8698
Shihezi University · CNFirst Affiliated Hospital of Shihezi University Medical College · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The occurrence of osteosarcoma (OS) is associated with abnormal expression of many microRNAs (miRNAs). Exosomal miRNAs get much more attentions in intracellular communications. miR-1307 has been studied in many cancers, but its effects in OS have not been studied. We hypothesized that OS-derived exosomal miR-1307 regulates OS tumorigenesis. First, we found OS cell-derived exosomes (Exos) significantly promoted the proliferation, migration, and invasion of OS cells. Secondly, we found miR-1307 was highly expressed in OS cell-derived exosomes (OS-Exos), human OS tissues, and OS cell lines. Then, OS-Exos were extracted after OS cells were cultured and transfected with miR-1307 inhibitor, and the level of miR-1307 in OS-Exos was significantly reduced. When the level of miR-1307 in OS-Exos was significantly reduced, the effects of OS-Exos on migration, invasion, and proliferation of OS cells were also significantly weakened. Furthermore, using TargetScan, miRDB, and mirDIP databases, we identified that AGAP1 was a target gene of miR-1307. Overexpression of miR-1307 could inhibit the expression of AGAP1 gene. We also found AGAP1 was lower expressed in human OS tissues and OS cell lines. Luciferase gene indicated that miR-1307 directly bound the 3'-UTR of AGAP1. miR-1307 was negatively correlated with AGAP1 in clinical study. miR-1307 could significantly promote the proliferation, migration, and invasion of OS cells. In addition, upregulation of AGAP1 could significantly inhibit the role of miR-1307 in OS. In conclusion, our study suggests that OS cell-derived exosomal miR-1307 promotes the proliferation, migration, and invasion of OS cells via targeting AGAP1, and miR-1307-AGAP1 axis may play an important role in the future treatment of OS.

Indexed as

3' Untranslated RegionsAdolescentAdultBase SequenceCarcinogenesisCell Line, TumorCell MovementCell ProliferationExosomesFemaleGene Expression Regulation, NeoplasticGTPase-Activating ProteinsHumansMaleMicroRNAsNeoplasm Invasiveness3' Untranslated RegionsAGAP1 protein, humanGTPase-Activating ProteinsMicroRNAsMIRN1307 microRNA, humanRNA, Messenger

Identifiers

PMID33834074
PMCPMC8016573
OpenAlexW3138637183

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.